MiR-126 suppresses colon cancer cell proliferation and invasion via inhibiting RhoA/ROCK signaling pathway

被引：52

作者：

Li, Nan ^{[1
,2
]}

Tang, Anliu ^{[1
,2
]}

Huang, Shuo ^{[1
]}

Li, Zeng ^{[1
]}

Li, Xiayu ^{[1
,2
]}

Shen, Shourong ^{[1
,2
]}

Ma, Jian ^{[2
,3
,4
,5
]}

Wang, Xiaoyan ^{[1
,2
]}

机构：

[1] Cent S Univ, Xiangya Hosp 3, Dept Gastroenterol, Changsha, Hunan, Peoples R China

[2] Hunan Key Lab Nonresolving Inflammat & Canc, Changsha, Hunan, Peoples R China

[3] Cent S Univ, Canc Res Inst, Changsha, Hunan, Peoples R China

[4] Minist Hlth, Key Lab Carcinogenesis, Changsha, Hunan, Peoples R China

[5] Minist Educ, Key Lab Carcinogenesis & Canc Invas, Changsha, Hunan, Peoples R China

来源：

MOLECULAR AND CELLULAR BIOCHEMISTRY | 2013年 / 380卷 / 1-2期

关键词：

MiR-126; Colon cancer; Cancer proliferation; Cancer metastasis; RhoA; RHO-GTPASES; BREAST-CANCER; COLORECTAL-CANCER; MICRORNA; EXPRESSION; METASTASIS; PROGNOSIS; CARCINOMA; MIGRATION; PROFILES;

D O I：

10.1007/s11010-013-1664-0

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Recent data strongly suggests the profound role of miRNAs in cancer progression. Here, we showed miR-126 expression was much lower in HCT116, SW620 and HT-29 colon cancer cells with highly metastatic potential and miR-126 downregulation was more frequent in colorectal cancers with metastasis. Restored miR-126 expression inhibited HT-29 cell growth, cell-cycle progression and invasion. Mechanically, microarray results combined with bioinformatic and experimental analysis demonstrated miR-126 exerted cancer suppressor role via inhibiting RhoA/ROCK signaling pathway. These results suggest miR-126 function as a potential tumor suppressor in colon cancer progression and miR-126/RhoA/ROCK may be a novel candidate for developing rational therapeutic strategies.

引用

页码：107 / 119

页数：13

共 46 条

[1]

Alwan A, 2007, DISASTER MED PUBLIC, V1, P7, DOI 10.1097/DMP.0b013e3180676d32

[2] Formation of actin stress fibers and focal adhesions enhanced by Rho-kinase [J].

Amano, M ;

Chihara, K ;

Kimura, K ;

Fukata, Y ;

Nakamura, N ;

Matsuura, Y ;

Kaibuchi, K .

SCIENCE, 1997, 275 (5304) :1308-1311

[3] MicroRNA pathways in flies and worms: Growth, death, fat, stress, and timing [J].

Ambros, V .

CELL, 2003, 113 (06) :673-676

[4] Rho GTPases in human cancer:: an unresolved link to upstream and downstream transcriptional regulation [J].

Benitah, SA ;

Valerón, PF ;

van Aelst, L ;

Marshall, CJ ;

Lacal, JC .

BIOCHIMICA ET BIOPHYSICA ACTA-REVIEWS ON CANCER, 2004, 1705 (02) :121-132

[5] bantam encodes a developmentally regulated microRNA that controls cell proliferation and regulates the proapoptotic gene hid in Drosophila [J].

Brennecke, J ;

Hipfner, DR ;

Stark, A ;

Russell, RB ;

Cohen, SM .

CELL, 2003, 113 (01) :25-36

[6] Protein kinase A gating of a pseudopodial-located RhoA/ROCK/p38/NHE1 signal module regulates invasion in breast cancer cell lines [J].

Cardone, RA ;

Bagorda, A ;

Bellizzi, A ;

Busco, G ;

Guerra, L ;

Paradiso, A ;

Casavola, V ;

Zaccolo, M ;

Reshkin, SJ .

MOLECULAR BIOLOGY OF THE CELL, 2005, 16 (07) :3117-3127

[7] Cell adhesion and signalling by cadherins and Ig-CAMs in cancer [J].

Cavallaro, U ;

Christofori, G .

NATURE REVIEWS CANCER, 2004, 4 (02) :118-132

[8] RhoA mediates cyclooxygenase-2 signaling to disrupt the formation of adherens junctions and increase cell motility [J].

Chang, Yu-Wen E. ;

Marlin, Jerry W. ;

Chance, Terry W. ;

Jakobi, Rolf .

CANCER RESEARCH, 2006, 66 (24) :11700-11708

[9] MicroRNAs modulate hematopoietic lineage differentiation [J].

Chen, CZ ;

Li, L ;

Lodish, HF ;

Bartel, DP .

SCIENCE, 2004, 303 (5654) :83-86

[10]

Cheng KW, 2009, CANCER TREAT RES, V149, P229, DOI 10.1007/978-0-387-98094-2_11

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