Endophytic fungi from Musa acuminata leaves and roots in South China

One hundred and sixty-three endophytic fungal cultures were isolated from 200 leaf samples of Musa acuminata trees, which were soaked in 36% formaldehyde solution for surface sterilization. They belonged to the genera of Gloeosporium musae (45%,), Myxosporium spp. (11%), Deightoniella torulosa (8.5%), Alternaria tennis (7.9%), Sphaceloma spp. (7.4%), Aureobasidium spp. (4.3%), Melida spp. (1.8%,), Uncinula spp. (1.8%), Penicillium spp. (1.8%), Aspergillus spp. (1.2%), Sarcinella spp. (1.2%), Cladosporium sp. (0.6%,), Cephalosporium sp. (0.6%) and sterile mycelium (6.7%). Sixty-eight endophytic fungal cultures were isolated from 100 root samples. They respectively belonged to the genera of Aspergillus spp. (31%), Paecilomyces spp. (16%), Penicillium spp. (15%), Fusarium spp. (10%), Gloeosporium musae (6%). yeast (3%), Deightoniella torulosa (3%,), Spicaria sp. (1.4%), Cephalosporium sp. (1.4%,), Meliola sp. (1.4%) and sterile mycelium (10%,). Water agar (containing 50 mug chloramphenicol ml(-1) and 50 mug streptomycin ml(-1)) seemed to be a better medium for isolation of endophytic fungi than potato-dextrose agar (PDA, containing 50 mug chloramphenicol ml(-1) and 50 mug streptomycin ml(-1)).