Real-time assays for the assembly-disassembly cycle of COP coats on liposomes of defined size

被引：25

作者：

Bigay, J ^{[1
]}

Antonny, B ^{[1
]}

机构：

[1] CNRS, Inst Pharmacol Mol & Cellulaire, Valbonne, France

来源：

GTPASES REGULATING MEMBRANE DYNAMICS | 2005年 / 404卷

关键词：

D O I：

10.1016/S0076-6879(05)04010-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The assembly-disassembly cycle of COPI and COPII coats is controlled by the GTPase cycle of the small G proteins Arf1 and Sar. We describe here two spectroscopic assays that enable real-time studies of some elementary steps of coat assembly and disassembly on artificial liposomes of defined composition and curvature. A flotation assay to assess the effect of membrane curvature on protein adsorption to liposomes is also presented.

引用

页码：95 / 107

页数：13

共 17 条

[1] N-terminal hydrophobic residues of the G-protein ADP-ribosylation factor-1 insert into membrane phospholipids upon GDP to GTP exchange [J].

Antonny, B ;

BeraudDufour, S ;

Chardin, P ;

Chabre, M .

BIOCHEMISTRY, 1997, 36 (15) :4675-4684

[2] Dynamics of the COPII coat with GTP and stable analogues [J].

Antonny, B ;

Madden, D ;

Hamamoto, S ;

Orci, L ;

Schekman, R .

NATURE CELL BIOLOGY, 2001, 3 (06) :531-537

[3] A glutamic finger in the guanine nucleotide exchange factor ARNO displaces Mg2+ and the β-phosphate to destabilize GDP on ARF1 [J].

Béraud-Dufour, S ;

Robineau, S ;

Chardin, P ;

Paris, S ;

Chabre, M ;

Cherfils, J ;

Antonny, B .

EMBO JOURNAL, 1998, 17 (13) :3651-3659

[4] Lipid packing sensed by ArfGAP1 couples COPI coat disassembly to membrane bilayer curvature [J].

Bigay, J ;

Gounon, P ;

Robineau, S ;

Antonny, B .

NATURE, 2003, 426 (6966) :563-566

[5] Coupling of coat assembly and vesicle budding to packaging of putative cargo receptors [J].

Bremser, M ;

Nickel, W ;

Schweikert, M ;

Ravazzola, M ;

Amherdt, M ;

Hughes, CA ;

Söllner, TH ;

Rothman, JE ;

Wieland, FT .

CELL, 1999, 96 (04) :495-506

[6]

Buser CA, 1998, METH MOL B, V84, P267

[7] GTP/GDP exchange by Sec12p enables COPII vesicle bud formation on synthetic liposomes [J].

Futai, E ;

Hamamoto, S ;

Orci, L ;

Schekman, R .

EMBO JOURNAL, 2004, 23 (21) :4146-4155

[8] Bi-directional protein transport between the ER and Golgi [J].

Lee, MCS ;

Miller, EA ;

Goldberg, J ;

Orci, L ;

Schekman, R .

ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY, 2004, 20 :87-123

[9] SMALL-VOLUME EXTRUSION APPARATUS FOR PREPARATION OF LARGE, UNILAMELLAR VESICLES [J].

MACDONALD, RC ;

MACDONALD, RI ;

MENCO, BPM ;

TAKESHITA, K ;

SUBBARAO, NK ;

HU, LR .

BIOCHIMICA ET BIOPHYSICA ACTA, 1991, 1061 (02) :297-303

[10] The use of liposomes to study COPII- and COPI-coated vesicle formation and membrane protein sorting [J].

Matsuoka, K ;

Schekman, R .

METHODS-A COMPANION TO METHODS IN ENZYMOLOGY, 2000, 20 (04) :417-428

← 1 2 →