Factors influencing the antioxidant activity determined by the ABTS(center dot+) radical cation assay

被引:551
作者
Miller, NJ [1 ]
RiceEvans, CA [1 ]
机构
[1] GUYS HOSP,UMDS,INT ANTIOXIDANT RES CTR,LONDON SE1 9RT,ENGLAND
关键词
ABTS(.+) radical cation; total antioxidant activity; flavonoid; ascorbic acid; Trolox;
D O I
10.3109/10715769709097799
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
This study introduces a simple direct antioxidant assay, based on the reduction of the ABTS(.+) radical cation, and compares it with the myoglobin/ABTS(.+) assay. The methods give closely similar results, establishing that the antioxidants studied to date in the latter assay act by scavenging the ABTS(.+) radical cation and not by inhibiting its formation through reduction of ferryl myoglobin or reaction with H2O2.
引用
收藏
页码:195 / 199
页数:5
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