Array CGH using whole genome amplification of fresh-frozen and formalin-fixed, paraffin-embedded tumor DNA

被引：70

作者：

Little, SE

Vuononvirta, R

Reis, JS

Natrajan, R

Iravani, M

Fenwick, K

Mackay, A

Ashworth, A

Pritchard-Jones, K

Jones, C ^{[1
]}

机构：

[1] Royal Marsden NHS Trust, Inst Canc Res, Sutton SM2 5NG, Surrey, England

[2] Inst Canc Res, Breakthrough Breast Canc Res Ctr, London SW3 6JB, England

来源：

GENOMICS | 2006年 / 87卷 / 02期

关键词：

amplification; array CGH; molecular pathology; paraffin; microdissection;

D O I：

10.1016/j.ygeno.2005.09.019

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The ability to utilize formalin-fixed, paraffin-embedded (FFPE) archival specimens reliably for high-resolution molecular genetic analysis would be of immense practical application in the study of human disease. We have evaluated the ability of the GenomePlex whole genome amplification (WGA) kit to amplify frozen and FFPE tissue for use in array CGH (aCGH). GenomePlex gave highly representative data compared with unamplified controls both from frozen material (Pearson's R-2 = 0.898) and from FFPE (R-2 = 0.883). Artifactual amplification observed using DOP-PCR at chromosomes 1p, 3, 13q, and 16p was not seen with GenomePlex. Highly reproducible aCGH profiles were obtained using as little as 5 ng starting material from FFPE (R-2 = 0.918). This WGA method should readily lend itself to the determination of DNA copy number alterations from small fresh-frozen and FFPE clinical tumor specimens, although some care must be taken to optimize the DNA extraction procedure. (C) 2005 Elsevier Inc. All rights reserved.

引用

页码：298 / 306

页数：9

共 30 条

[1] Chromosome aberrations in solid tumors [J].