Untransformed root cultures of Nothapodytes foetida and production of camptothecin

被引：22

作者：

Fulzele, DP ^{[1
]}

Satdive, RK ^{[1
]}

Pol, BB ^{[1
]}

机构：

[1] Bhabha Atom Res Ctr, Plant Biotechnol & Secondary Prod Sect, Nucl Agr & Biotechnol Div, Mumbai 400085, India

来源：

PLANT CELL TISSUE AND ORGAN CULTURE | 2002年 / 69卷 / 03期

关键词：

camptothecin; 9-methoxy camptothecin; Nothapodytes foetida; untransformed root cultures;

D O I：

10.1023/A:1015657927304

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Untransformed root cultures of Nothapodytes foetida were established from immature zygotic embryos on MS basal medium supplemented with different concentration of growth regulators. Alkaloid contents in untransformed root cultures showed that root elongation and growth regulators accompanied product synthesis. Basal medium supplemented with NAA and BA achieved maximum number of elongated roots. Maximum concentration of camptothecin (0.01% DW) and 9-methoxy camptothecin (0.0016% DW) were synthesised by untransformed root cultures incubated on MS medium supplemented with NAA (71.36 muM) and BA (8.87 muM). Culture medium containing NAA (71.36 muM) and Kn (9.29 muM) proliferated callus interspersed with roots which synthesised camptothecin (0.00017% DW) and 9-methoxy-camptothecin (0.000058% DW).

引用

页码：285 / 288

页数：4

共 14 条

[11] PRODUCTION OF BISBENZYLISOQUINOLINE ALKALOIDS IN CULTURED ROOTS OF STEPHANIA-CEPHARANTHA [J].