Role of the Ser-287-Asn Replacement in the Hydrolysis Spectrum Extension of AmpC β-Lactamases in Escherichia coli

被引：20

作者：

Mammeri, Hedi ^{[1
,2
,3
]}

Galleni, Moreno ^{[4
]}

Nordmann, Patrice ^{[2
,3
]}

机构：

[1] CHU Amiens, Hop Nord, Serv Bacteriol Hyg, F-8000 Amiens, France

[2] Hop Bicetre, AP HP, Unite INSERM Emerging Resistance Antibiot 914, Serv Bacteriol Virol Hyg,Fac Med, F-94275 Le Kremlin Bicetre, France

[3] Univ Paris 11, F-94275 Le Kremlin Bicetre, France

[4] Univ Liege, Ctr Prot Engn, B-4000 Cointe Ougree, Belgium

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2009年 / 53卷 / 01期

关键词：

SWISS-MODEL; CEPHALOSPORINASES;

D O I：

10.1128/AAC.00608-08

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Two AmpC variants harboring the S287N substitution were obtained by mutagenesis from cephalosporinases representative of the phylogenetic groups A and B2 of Escherichia coli. Their biochemical characterization revealed that the S287N replacement led to an important increase in the catalytic efficiency toward extended-spectrum cephalosporins in the AmpC beta-lactamase of group A only.

引用

页码：323 / 326

页数：4

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