High pressure static fluorescence to study macromolecular structure-function

被引：31

作者：

Ruan, K ^{[1
]}

Balny, C ^{[1
]}

机构：

[1] CNRS, INSERM, U 128, IFR 24,Inst Natl Sante Rech Med, F-34293 Montpellier 5, France

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY | 2002年 / 1595卷 / 1-2期

关键词：

fluorescence; conformational change; enzymatic activity; fluorescence polarization; stopped-flow; high hydrostatic pressure;

D O I：

10.1016/S0167-4838(01)00337-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Through some typical examples, the high pressure static fluorescence method is described. The potentiality of the intrinsic and extrinsic fluorescence probes are analyzed for structural characterizations. Special attention is given to the use of fluorescence to understand the behavior of enzymatic reactions under high pressure. The application of fluorescence polarization is also presented together with some relevant spectroscopic problems inherent in data interpretation. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：94 / 102

页数：9

共 44 条

[31] A stable partly denatured state of trypsin induced by high hydrostatic pressure [J].

Ruan, KC ;

Lange, R ;

Bec, N ;

Balny, C .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1997, 239 (01) :150-154

[32] DISSOCIATION OF YEAST HEXOKINASE BY HYDROSTATIC-PRESSURE [J].

RUAN, KC ;

WEBER, G .

BIOCHEMISTRY, 1988, 27 (09) :3295-3301

[33] Unusual effect of high hydrostatic pressure on basic phospholipase A2 from venom of Agkistrodon Halys Pallas [J].

Ruan, KC ;

Lange, R ;

Zhou, YC ;

Balny, C .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1998, 249 (03) :844-848

[34] Pressure effects on tryptophan and its derivatives [J].

Ruan, KC ;

Tian, SM ;

Lange, R ;

Balny, C .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2000, 269 (03) :681-686

[35] Pressure-exploration of the 33-kDa protein from the spinach photosystem II particle [J].

Ruan, KC ;

Xu, CH ;

Yu, Y ;

Li, J ;

Lange, R ;

Bec, N ;

Balny, C .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 2001, 268 (09) :2742-2750

[36] PHYSICAL HETEROGENEITY OF MUSCLE GLYCOGEN-PHOSPHORYLASE REVEALED BY HYDROSTATIC-PRESSURE DISSOCIATION [J].

RUAN, KC ;

WEBER, G .

BIOCHEMISTRY, 1993, 32 (24) :6295-6301

[37]

SILVA JL, 1993, ANNU REV PHYS CHEM, V44, P89, DOI 10.1146/annurev.pc.44.100193.000513

[38]

SILVA JL, 1986, BIOCHEMISTRY-US, V25, P5781

[39] Pressure effect on the conformational fluctuation of apomyoglobin in the native state [J].

Tanaka, N ;

Ikeda, C ;

Kanaori, K ;

Hiraga, K ;

Konno, T ;

Kunugi, S .

BIOCHEMISTRY, 2000, 39 (39) :12063-12068

[40]

TANG GQ, 1995, ACTA BIOCHIM BIOPHYS, V27, P113

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