Molecular characterization of the 71E late puff in Drosophila melanogaster reveals a family of novel genes

被引:40
作者
Wright, LG
Chen, TH
Thummel, CS
Guild, GM
机构
[1] UNIV PENN,DEPT BIOL,PHILADELPHIA,PA 19104
[2] UNIV UTAH,HOWARD HUGHES MED INST,DEPT HUMAN GENET,ECCLES INST HUMAN GENET 5200,SALT LAKE CITY,UT 84112
关键词
Drosophila; ecdysone; metamorphosis; chromosome puff; gene family;
D O I
10.1006/jmbi.1996.0032
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Early metamorphic development in Drosophila melanogaster is initiated by pulses of the steroid hormone ecdysone, which are transduced into tissue-specific transcriptional cascades. This process begins with the hormone-dependent activation of a set of transcription factors (early genes) that, in turn, activate set of tissue-specific effector genes (late genes). The 71E cytogenetic region of the salivary gland polytene genome contains several ecdysone-regulated transcription units. Molecular techniques were used to analyze these genes, their transcriptional program and their evolutionary relatedness. We find that this region contains a cluster of ten coordinately regulated late genes (L71 genes) that are organized as five divergently transcribed gene pairs. Maximum parsimony analysis suggests that an ancestral L71 gene duplicated to form the first gene pair which was, in turn, duplicated to form the set of gene pairs. The L71 gene products form a family of small, chemically basic proteins with a conserved backbone of cysteine residues. In addition, the 71E region contains another gene (I71-1) with the regulatory and biochemical characteristics of the salivary gland intermolt glue proteins. (C) 1996 Academic Press Limited
引用
收藏
页码:387 / 400
页数:14
相关论文
共 50 条
[41]   3' NON-CODING REGION SEQUENCES IN EUKARYOTIC MESSENGER-RNA [J].
PROUDFOOT, NJ ;
BROWNLEE, GG .
NATURE, 1976, 263 (5574) :211-214
[42]   AN ECDYSTERONE-RESPONSIVE PUFF SITE IN DROSOPHILA CONTAINS A CLUSTER OF 7 DIFFERENTIALLY REGULATED GENES [J].
RESTIFO, LL ;
GUILD, GM .
JOURNAL OF MOLECULAR BIOLOGY, 1986, 188 (04) :517-528
[43]   POLY(A) SHORTENING OF COREGULATED TRANSCRIPTS IN DROSOPHILA [J].
RESTIFO, LL ;
GUILD, GM .
DEVELOPMENTAL BIOLOGY, 1986, 115 (02) :507-510
[44]   DROSOPHILA SALIVARY-GLAND PROTEINS AND PUPATION [J].
SARMIENTO, LA ;
MITCHELL, HK .
DEVELOPMENTAL GENETICS, 1982, 3 (03) :255-272
[45]   ELECTRON MICROSCOPICAL ANALYSIS OF DROSOPHILA POLYTENE CHROMOSOMES .2. DEVELOPMENT OF COMPLEX PUFFS [J].
SEMESHIN, VF ;
BARICHEVA, EM ;
BELYAEVA, ES ;
ZHIMULEV, IF .
CHROMOSOMA, 1985, 91 (3-4) :210-233
[46]   THE DROSOPHILA 78C EARLY-LATE PUFF CONTAINS E78, AN ECDYSONE-INDUCIBLE GENE THAT ENCODES A NOVEL MEMBER OF THE NUCLEAR HORMONE-RECEPTOR SUPERFAMILY [J].
STONE, BL ;
THUMMEL, CS .
CELL, 1993, 75 (02) :307-320
[47]  
SWOFFORD DL, 1993, PAUP PHYLOGENETIC AN
[48]  
URNESS LD, 1995, IN PRESS EMBO J
[49]   CYTOGENETIC AND GENETIC-MAPPING OF A SALIVARY-GLAND SECRETION PROTEIN IN DROSOPHILA-MELANOGASTER [J].
VELISSARIOU, V ;
ASHBURNER, M .
CHROMOSOMA, 1981, 84 (02) :173-185
[50]  
VONHEIJNE G, 1983, EUR J BIOCHEM, V133, P17