Membrane fission by dynamin: what we know and what we need to know

被引：358

作者：

Antonny, Bruno ^{[1
]}

Burd, Christopher ^{[2
]}

De Camilli, Pietro ^{[3
,4
,5
]}

Chen, Elizabeth ^{[6
]}

Daumke, Oliver ^{[7
]}

Faelber, Katja ^{[7
]}

Ford, Marijn ^{[8
]}

Frolov, Vadim A. ^{[9
,10
,11
]}

Frost, Adam ^{[12
]}

Hinshaw, Jenny E. ^{[13
]}

Kirchhausen, Tom ^{[14
,15
,16
]}

Kozlov, Michael M. ^{[17
]}

Lenz, Martin ^{[18
]}

Low, Harry H. ^{[19
]}

McMahon, Harvey ^{[20
]}

Merrifield, Christien ^{[21
]}

Pollard, Thomas D. ^{[22
]}

Robinson, Phillip J. ^{[23
]}

Roux, Aurelien ^{[24
,25
]}

Schmid, Sandra ^{[26
]}

机构：

[1] Univ Nice Sophia Antipolis, CNRS, Inst Pharmacol Mol & Cellulaire, Valbonne, France

[2] Yale Univ, Sch Med, Dept Cell Biol, New Haven, CT 06510 USA

[3] Yale Univ, Sch Med, Howard Hughes Med Inst, Dept Neurosci, New Haven, CT 06510 USA

[4] Yale Univ, Sch Med, Howard Hughes Med Inst, Dept Cell Biol, New Haven, CT 06510 USA

[5] Yale Univ, Sch Med, Kavli Inst Neurosci, New Haven, CT USA

[6] Univ Texas Southwestern Med Ctr Dallas, Dept Mol Biol, Dallas, TX 75390 USA

[7] Max Delbruck Ctr Mol Med, Dept Crystallog, Berlin, Germany

[8] Univ Pittsburgh, Sch Med, Dept Cell Biol, Pittsburgh, PA USA

[9] Univ Basque Country, CSIC, Biofis Inst, Leioa, Spain

[10] Univ Basque Country, Dept Biochem & Mol Biol, Leioa, Spain

[11] Basque Fdn Sci, Ikerbasque, Bilbao, Spain

[12] Univ Calif San Francisco, Dept Biochem & Biophys, San Francisco, CA 94143 USA

[13] NIDDK, Lab Cell & Mol Biol, NIH, Bethesda, MD 20892 USA

[14] Harvard Med Sch, Dept Cell Biol, Boston, MA USA

[15] Harvard Med Sch, Dept Pediat, Boston, MA USA

[16] Boston Childrens Hosp, Program Cellular & Mol Med, Boston, MA USA

[17] Tel Aviv Univ, Sackler Fac Med, Dept Physiol & Pharmacol, Tel Aviv, Israel

[18] Univ Paris Saclay, Univ Paris Sud, CNRS, LPTMS, Orsay, France

[19] Imperial Coll, Dept Life Sci, London, England

[20] MRC Lab Mol Biol, Cambridge, England

[21] Inst Integrat Biol Cell, Gif Sur Yvette, France

[22] Yale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT USA

[23] Univ Sydney, Childrens Med Res Inst, Cell Signalling Unit, Westmead, NSW, Australia

[24] Univ Geneva, Dept Biochem, Geneva 4, Switzerland

[25] Univ Geneva, Swiss NCCR Chem Biol, Geneva 4, Switzerland

[26] Univ Texas Southwestern Med Ctr Dallas, Dept Cell Biol, Dallas, TX 75390 USA

来源：

EMBO JOURNAL | 2016年 / 35卷 / 21期

基金：

英国医学研究理事会;

关键词：

dynamin; membrane fission; endocytosis; GTPase; molecular motor; CLATHRIN-MEDIATED ENDOCYTOSIS; SYNAPTIC VESICLE ENDOCYTOSIS; DEPENDENT CONFORMATIONAL-CHANGES; DOMAIN-CONTAINING PROTEINS; MITOCHONDRIAL FISSION; CRYSTAL-STRUCTURE; GTP HYDROLYSIS; BAR DOMAINS; COATED-PIT; MECHANOCHEMICAL ENZYME;

D O I：

10.15252/embj.201694613

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

The large GTPase dynamin is the first protein shown to catalyze membrane fission. Dynamin and its related proteins are essential to many cell functions, from endocytosis to organelle division and fusion, and it plays a critical role in many physiological functions such as synaptic transmission and muscle contraction. Research of the past three decades has focused on understanding how dynamin works. In this review, we present the basis for an emerging consensus on how dynamin functions. Three properties of dynamin are strongly supported by experimental data: first, dynamin oligomerizes into a helical polymer; second, dynamin oligomer constricts in the presence of GTP; and third, dynamin catalyzes membrane fission upon GTP hydrolysis. We present the two current models for fission, essentially diverging in how GTP energy is spent. We further discuss how future research might solve the remaining open questions presently under discussion.

引用

页码：2270 / 2284

页数：15

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