Aptamer-Based Viability Impedimetric Sensor for Bacteria

被引：119

作者：

Labib, Mahmoud ^{[1
]}

Zamay, Anna S. ^{[3
]}

Koloyskaya, Olga S. ^{[2
]}

Reshetneva, Irina T. ^{[3
]}

Zamay, Galina S. ^{[2
]}

Kibbee, Richard J. ^{[4
]}

Sattar, Syed A. ^{[4
]}

Zamay, Tatiana N. ^{[2
]}

Berezovski, Maxim V. ^{[1
]}

机构：

[1] Univ Ottawa, Dept Chem, Ottawa, ON K1N 6N5, Canada

[2] Krasnoyarsk State Med Univ, Inst Mol Med & Pathol Biochem, Krasnoyarsk 660022, Russia

[3] Krasnoyarsk State Med Univ, Dept Microbiol, Krasnoyarsk 660022, Russia

[4] Univ Ottawa, Fac Med, Ctr Res Environm Microbiol, Ottawa, ON K1H 8M5, Canada

来源：

ANALYTICAL CHEMISTRY | 2012年 / 84卷 / 21期

基金：

加拿大自然科学与工程研究理事会;

关键词：

SALMONELLA; BIOSENSOR;

D O I：

10.1021/ac302902s

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The development of an aptamer-based viability impedimetric sensor for bacteria (AptaVISens-B) is presented. Highly specific DNA aptamers to live Salmonella typhimurium were selected via the cell-systematic evolution of ligands by exponential enrichment (SELEX) technique. Twelve rounds of selection were performed; each comprises a positive selection step against viable S. typhimurium and a negative selection step against heat killed S. typhimurium and a mixture of related pathogens, including Salmonella enteritidis, Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Citrobacter freundii to ensure the species specificity of the selected aptamers. The DNA sequence showing the highest binding affinity to the bacteria was further integrated into an impedimetric sensor via self assembly onto a gold nanoparticle-modified screen printed carbon electrode (GNP-SPCE). Remarkably, this aptasensor is highly selective and can successfully detect S. typhimurium down to 600 CFU mL(-1) (equivalent to 18 live cells in 30 mu L of assay volume) and distinguish it from other Salmonella species, including S. enteritidis and S. choleraesuis. This report is envisaged to open a new venue for the aptamer-based viability sensing of a variety of microorganisms, particularly viable but nonculturable (VBNC) bacteria, using a rapid, economic, and label-free electrochemical platform.

引用

页码：8966 / 8969

页数：4

共 22 条

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