A roadmap for interpreting 13C metabolite labeling patterns from cells

被引：452

作者：

Buescher, Joerg M. ^{[1
,2
]}

Antoniewicz, Maciek R. ^{[3
]}

Boros, Laszlo G. ^{[4
,5
]}

Burgess, Shawn C. ^{[6
,7
]}

Brunengraber, Henri ^{[8
]}

Clish, Clary B. ^{[9
]}

DeBerardinis, Ralph J. ^{[10
]}

Feron, Olivier ^{[11
]}

Frezza, Christian ^{[12
]}

Ghesquiere, Bart ^{[1
,2
]}

Gottlieb, Eyal ^{[13
]}

Hiller, Karsten ^{[14
]}

Jones, Russell G. ^{[15
]}

Kamphorst, Jurre J. ^{[16
]}

Kibbey, Richard G. ^{[17
]}

Kimmelman, Alec C. ^{[18
]}

Locasale, Jason W. ^{[19
]}

Lunt, Sophia Y. ^{[20
]}

Maddocks, Oliver D. K. ^{[13
]}

Malloy, Craig ^{[6
,21
]}

Metallo, Christian M. ^{[22
]}

Meuillet, Emmanuelle J. ^{[23
,24
,25
]}

Munger, Joshua ^{[26
,27
]}

Noeh, Katharina ^{[28
]}

Rabinowitz, Joshua D. ^{[29
,30
]}

Ralser, Markus ^{[31
,32
,33
]}

Sauer, Uwe ^{[34
]}

Stephanopoulos, Gregory ^{[35
]}

St-Pierre, Julie ^{[36
,37
]}

Tennant, Daniel A. ^{[38
]}

Wittmann, Christoph ^{[39
]}

Vander Heiden, Matthew G. ^{[40
,41
]}

Vazquez, Alexei ^{[13
]}

Vousden, Karen ^{[13
]}

Young, Jamey D. ^{[42
,43
]}

Zamboni, Nicola ^{[34
]}

Fendt, Sarah-Maria ^{[1
,2
]}

机构：

[1] VIB, Vesalius Res Ctr, Leuven, Belgium

[2] Katholieke Univ Leuven, Dept Oncol, Leuven, Belgium

[3] Univ Delaware, Dept Chem & Biomol Engn, Newark, DE USA

[4] Univ Calif Los Angeles, Sch Med, Dept Pediat, Los Angeles Biomed Res Inst,Harbor UCLA Med Ctr, Los Angeles, CA 90024 USA

[5] Sidmap LLC, Los Angeles, CA USA

[6] Univ Texas SW Med Ctr Dallas, Adv Imaging Res Ctr, Div Metab Mech Dis, Dallas, TX 75390 USA

[7] Univ Texas SW Med Ctr Dallas, Dept Pharmacol, Dallas, TX 75390 USA

[8] Case Western Reserve Univ, Sch Med, Dept Nutr, Cleveland, OH USA

[9] Broad Inst Harvard & MIT, Cambridge, MA USA

[10] Univ Texas SW Med Ctr Dallas, Childrens Med Ctr, Res Inst, Dallas, TX 75390 USA

[11] Catholic Univ Louvain, Pole Pharmacol & Therapeut FATH, IREC, B-1200 Brussels, Belgium

[12] Univ Cambridge, Hutchison MRC Res Ctr, MRC Canc Unit, Cambridge, England

[13] Canc Res UK, Beatson Inst, Glasgow, Lanark, Scotland

[14] Univ Luxembourg, Luxembourg Ctr Syst Biomed, Esch Belval, Luxembourg

[15] McGill Univ, Dept Physiol, Goodman Canc Res Ctr, Montreal, PQ, Canada

[16] Univ Glasgow, Inst Canc Sci, Glasgow, Lanark, Scotland

[17] Yale Univ, Sch Med, Internal Med Cellular & Mol Physiol, New Haven, CT USA

[18] Dana Farber Canc Inst, Dept Radiat Oncol, Div Genom Stabil & DNA Repair, Boston, MA 02115 USA

[19] Cornell Univ, Div Nutr Sci, Ithaca, NY 14853 USA

[20] Michigan State Univ, Dept Biochem & Mol Biol, E Lansing, MI 48824 USA

[21] Univ Texas SW Med Ctr Dallas, Dept Radiol, Dallas, TX 75390 USA

[22] Univ Calif San Diego, Dept Bioengn, La Jolla, CA 92093 USA

[23] Inst Technol Avancees Sci Vivant ITAV, Toulouse 1, France

[24] Univ Arizona, Ctr Canc, Tucson, AZ USA

[25] Univ Arizona, Dept Nutr Sci, Tucson, AZ USA

[26] Univ Rochester, Med Ctr, Dept Biochem, Rochester, NY 14642 USA

[27] Univ Rochester, Med Ctr, Dept Biophys, Rochester, NY 14642 USA

[28] Forschungszentrum Julich, Inst Bio & Geosci, IBG Biotechnol 1, D-52425 Julich, Germany

[29] Princeton Univ, Dept Chem, Princeton, NJ 08544 USA

[30] Princeton Univ, Lewis Sigler Inst Integrat Genom, Princeton, NJ 08544 USA

[31] Univ Cambridge, Cambridge Syst Biol Ctr, Cambridge, England

[32] Univ Cambridge, Dept Biochem, Cambridge CB2 1QW, England

[33] MRC Natl Inst Med Res, Div Physiol & Metab, London, England

[34] ETH, Inst Mol Syst Biol, Zurich, Switzerland

[35] MIT, Dept Chem Engn, Cambridge, MA 02139 USA

[36] McGill Univ, Goodman Canc Res Ctr, Montreal, PQ, Canada

[37] McGill Univ, Dept Biochem, Montreal, PQ, Canada

[38] Univ Birmingham, Sch Canc Sci, Coll Med & Dent Sci, Birmingham, W Midlands, England

[39] Univ Saarland, Inst Syst Biotechnol, D-66123 Saarbrucken, Germany

[40] MIT, Broad Inst Harvard & MIT, Koch Inst Integrat Canc Res, Cambridge, MA 02139 USA

[41] Dana Farber Canc Inst, Dept Med Oncol, Boston, MA 02115 USA

[42] Vanderbilt Univ, Dept Chem & Biomol Engn, Nashville, TN 37235 USA

[43] Vanderbilt Univ, Dept Mol Physiol & Biophys, Nashville, TN 37232 USA

来源：

CURRENT OPINION IN BIOTECHNOLOGY | 2015年 / 34卷

关键词：

REDUCTIVE GLUTAMINE-METABOLISM; MASS ISOTOPOMER DISTRIBUTIONS; PENTOSE-PHOSPHATE PATHWAY; FATTY-ACID SYNTHESIS; FLUX ANALYSIS; CORYNEBACTERIUM-GLUTAMICUM; ALPHA-KETOGLUTARATE; TCA CYCLE; RAT-LIVER; GROWTH;

D O I：

10.1016/j.copbio.2015.02.003

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Measuring intracellular metabolism has increasingly led to important insights in biomedical research. C-13 tracer analysis, although less information-rich than quantitative C-13 flux analysis that requires computational data integration, has been established as a time-efficient method to unravel relative pathway activities, qualitative changes in pathway contributions, and nutrient contributions. Here, we review selected key issues in interpreting C-13 metabolite labeling patterns, with the goal of drawing accurate conclusions from steady state and dynamic stable isotopic tracer experiments.

引用

页码：189 / 201

页数：13

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