Role of transmembrane 4 superfamily (TM4SF) proteins CD9 and CD81 in muscle cell fusion and myotube maintenance

被引:188
作者
Tachibana, I
Hemler, ME
机构
[1] Dana Farber Canc Inst, Boston, MA 02115 USA
[2] Harvard Univ, Sch Med, Boston, MA USA
关键词
TM4SF proteins; CD9; CD81; myoblast; myotube;
D O I
10.1083/jcb.146.4.893
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The role of transmembrane 4 superfamily (TM4SF) proteins during muscle cell fusion has not been investigated previously, Here we show that the appearance of TM4SF protein, CD9, and the formation of CD9-beta 1 integrin complexes were both regulated in coordination with murine C2C12 myoblast cell differentiation. Also, anti-CD9 and anti-CD81 monoclonal antibodies substantially inhibited and delayed conversion of C2C12 cells to elongated myotubes, without affecting muscle-specific protein expression. Studies of the human myoblast-derived RD sarcoma cell line further demonstrated that TM4SF proteins have a role during muscle cell fusion. Ectopic expression of CD9 caused a four- to eightfold increase in RD cell syncytia formation, whereas anti-CD9 and anti-CD81 antibodies markedly delayed RD syncytia formation. Finally, anti-CD9 and anti-CD81 monoclonal antibodies triggered apoptotic degeneration of C2C12 cell myotubes after they were formed. In summary,TM4SF proteins such as CD9 and CD81 appear to promote muscle cell fusion and support myotube maintenance.
引用
收藏
页码:893 / 904
页数:12
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