Comparison of ELISA and capillary electrophoresis with laser-induced fluorescence detection in the analysis of Ochratoxin A in low volumes of human blood serum

被引:25
作者
Koeller, Gdbor
Wichmann, Gunnar
Rolle-Kampczyk, Ulrike
Popp, Peter
Herbarth, Olf
机构
[1] UFZ Helmholtz Ctr Environm Res, Dept Human Exposure Res & Epidemiol, D-04318 Leipzig, Germany
[2] UFZ Helmholtz Ctr Environm Res, Dept Environm Immunol, D-04318 Leipzig, Germany
[3] UFZ Helmholtz Ctr Environm Res, Dept Analyt Chem, D-04318 Leipzig, Germany
来源
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2006年 / 840卷 / 02期
关键词
capillary electrophoresis; CE-LIF human blood serum; low sample volume; Ochratoxin A; ELISA;
D O I
10.1016/j.jchromb.2006.04.048
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
In this paper the determination of Ochratoxin A (OTA) in low volumes of human blood serum by enzyme-linked immunosorbent assay (ELISA) is compared with an appropriate capillary electrophoresis with laser-induced fluorescence detection (CE-LIF) method. In order to use ELISA for high-throughput analysis in epidemiological studies no sample cleanup was performed. Both methods showed a limit of detection (LOD) of 0.5 ng/mL. Comparing the precisions of both methods, the data show that the quantified concentrations in ELISA are higher than the corresponding concentrations in the CE-LIF method. Using a matrix calibration curve instead of a standard calibration curve the reproducibilities of both methods are comparable. No additional matrix effect could be observed by adding phenylalanine as probable matrix compound to the serum. (c) 2006 Elsevier B.V. All rights reserved.
引用
收藏
页码:94 / 98
页数:5
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