SCP and crude pectinase production by slurry-state fermentation of lemon pulps

被引:65
作者
De Gregorio, A [1 ]
Mandalari, G [1 ]
Arena, N [1 ]
Nucita, F [1 ]
Tripodo, MM [1 ]
Lo Curto, RB [1 ]
机构
[1] Univ Messina, Dept Organ & Biol Chem, I-98166 Messina, Italy
关键词
slurry-state fermentation; SCP; Aspergillus niger; Trichoderma viride; crude pectinase; polygalacturonase;
D O I
10.1016/S0960-8524(01)00209-7
中图分类号
S2 [农业工程];
学科分类号
0828 ;
摘要
Single cell protein (SCP) and crude pectinolytic enzymes production from citrus pulps is reported. SCP and enzymes were produced by slurry-state flask cultivation of Aspergillus niger and Trichoderma viride on pulps from lemon juice clarification. Production as well as crude pectinase activity was not affected by the high dry matter content of the pulps. Both the protein content in the residue and the enzyme activity in the supernatant were higher in T. viride than in A. niger culture. The crude pectinase of T. viride, whose specific activity was similar to that found for a commercial concentrated preparation, could be utilized in the same citrus processing factory as well as in other factories which use large amounts of pectinolytic crude preparations, for example to enhance depuration plant performance. (C) 2002 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:89 / 94
页数:6
相关论文
共 38 条
[1]  
ABDELFATTAH AF, 1981, UTILIZATION CANE SUG, P388
[2]   EFFECT OF WATER ACTIVITY ON EXO-PECTINASE PRODUCTION BY ASPERGILLUS-NIGER CH4 ON SOLID-STATE FERMENTATION [J].
ACUNAARGUELLES, M ;
GUTIERREZROJAS, M ;
VINIEGRAGONZALEZ, G ;
FAVELATORRES, E .
BIOTECHNOLOGY LETTERS, 1994, 16 (01) :23-28
[3]   Effect of alkaline treatments at various temperatures on cellulase and biomass production using submerged sugarcane bagasse fermentation with Trichoderma reesei QM 9414 [J].
Aiello, C ;
Ferrer, A ;
Ledesma, A .
BIORESOURCE TECHNOLOGY, 1996, 57 (01) :13-18
[4]  
Al-Obaidi Z., 1987, J AGR WATER RESOUR R, V6, P125
[5]   CELLULASE METHODS FOR EFFICIENT DIGESTION OF GRASSES AND BRASSICAS [J].
ALLISON, M ;
BORZUCKI, R .
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, 1978, 29 (03) :293-297
[6]  
[Anonymous], 1997, OFFICIAL METHODS ANA
[7]   ENDOPOLYGALACTURONASE PRODUCTION FROM KLUYVEROMYCES-MARXIANUS .1. RESOLUTION, PURIFICATION, AND PARTIAL CHARACTERIZATION OF THE ENZYME [J].
BARNBY, FM ;
MORPETH, FF ;
PYLE, DL .
ENZYME AND MICROBIAL TECHNOLOGY, 1990, 12 (11) :891-897
[8]  
BEROVIC M, 1993, J CHEM TECHNOL BIOT, V56, P209
[9]   Recovery of pectolytic enzymes produced by solid state culture of Aspergillus niger [J].
Castilho, LR ;
Alves, TLM ;
Medronho, RA .
PROCESS BIOCHEMISTRY, 1999, 34 (02) :181-186
[10]   Production and extraction of pectinases obtained by solid state fermentation of agroindustrial residues with Aspergillus niger [J].
Castilho, LR ;
Medronho, RA ;
Alves, TLM .
BIORESOURCE TECHNOLOGY, 2000, 71 (01) :45-50