Chemical composition and anti-inflammatory activity of pectic polysaccharide isolated from celery stalks

被引：107

作者：

Ovodova, Raisa G. ^{[1
]}

Golovchenko, Victoria V. ^{[1
]}

Popov, Sergey V. ^{[1
]}

Popova, Galina Yu. ^{[1
]}

Paderin, Nikita M. ^{[1
]}

Shashkov, Alexandre S. ^{[2
]}

Ovodov, Yury S. ^{[1
]}

机构：

[1] Russian Acad Sci, Inst Physiol, Komi Sci Ctr, Urals Branch, Syktyvkar 167982, Russia

[2] Russian Acad Sci, ND Zelinskii Organ Chem Inst, Moscow 119991, Russia

来源：

FOOD CHEMISTRY | 2009年 / 114卷 / 02期

关键词：

Apium graveolens var. Dulce; Pectin; Structural features; Apiuman; Pectinase digestion; NMR-spectroscopy of polysaccharides; Anti-inflammatory activity; C-REACTIVE PROTEIN; COMARUM-PALUSTRE; DIETARY FIBER; INFLAMMATION; HYPERTENSION; ASSOCIATION; STRESS; HEALTH; MICE; L;

D O I：

10.1016/j.foodchem.2008.09.094

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A pectic polysaccharide called apiuman was isolated from fresh celery stalks by extraction with an aqueous ammonium oxalate followed by ultrafiltration, and was shown to consist Of D-galacturonic acid (GalA, 81.0%), L-rhamnose (Rha, 2.6%), L-arabinose (Ara, 2.5%), and D-galactose (Gal, 3.6%) residues. Apiuman was found to be digestible with alpha-1,4-D-galacturonase to yield D-GalA, thus confirming that apiuman represents a pectic polysaccharide. The purified apiuman (AG) obtained was subjected to ion exchange chromatography on DEAE-cellulose to produce two polysaccharide fractions, AG-1 and AG-2, which had similar sugar compositions. Partial acid hydrolysis of apiuman, AG, revealed galacturonan to be at the core of the macromolecule. Nuclear magnetic resonance (NMR) spectra indicated that the backbone of apiuman appeared to represent alpha-1,4-D-galacturonan, with considerable amounts of L-Rha residues involved by alpha-1,2-linkages in the linear chain of the macromolecule core. The side chains were found to consist of Ara and Gal attached at the 4-position of the Rha residues. Furthermore, apiuman. AG, was found to improve the survival of mice subjected to a lethal dose of lipopolysaccharide (LPS), and the anti-endotoxemic effect of apiuman, AG, was shown to be mediated by decreased interleukin-1 beta (IL-1 beta and increased interleukin-10 (IL-10) production. Apiuman was also shown to diminish the amount of neutrophils migrating to the peritoneal cavity after LPS injection. (c) 2008 Elsevier Ltd. All rights reserved.

引用

页码：610 / 615

页数：6

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