Cloning and expression of two fragments of the S gene of canine coronavirus type I

被引：11

作者：

Pratelli, A ^{[1
]}

Elia, G ^{[1
]}

Decaro, N ^{[1
]}

Tola, S ^{[1
]}

Tinelli, A ^{[1
]}

Martella, V ^{[1
]}

Rocca, S ^{[1
]}

Tempesta, M ^{[1
]}

Buonavoglia, C ^{[1
]}

机构：

[1] Univ Bari, Fac Vet Med, Dept Anim Hlth & Well Being, I-70010 Bari, Italy

来源：

JOURNAL OF VIROLOGICAL METHODS | 2004年 / 117卷 / 01期

关键词：

canine coronavirus; genotype I; recombinant S protein;

D O I：

10.1016/j.jviromet.2003.12.003

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Two fragments, S66 and S55, of the S glycoprotein of the newly identified canine coronavirus type I (CCoV type I), were expressed in a procariotic system. The purified recombinant proteins of 350 and 366 amino acids in length, respectively, were employed to develop an enzyme-linked immunosorbent assay (ELISA) for the detection of CCoV type I antibodies in dog sera. Four canine sera-positive for CCoV type II, four sera-positive for CCoV type I and 10 negative control sera were examined. Only the sera-positive for CCoV type I strongly reacted with both the proteins, whereas the sera-positive for CCoV type II showed low reactivity in the ELISA test. As CCoV type I seems to be not cultivable in cell cultures, the recombinant fragments of the S protein represent a unique method to study, preliminarily, the immunological and the pathogenetic characteristics of this new virus. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：61 / 65

页数：5

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