Comprehensive profiling of circulating microRNA via small RNA sequencing of cDNA libraries reveals biomarker potential and limitations

被引：283

作者：

Williams, Zev ^{[1
,2
,3
,4
]}

Ben-Dov, Iddo Z. ^{[2
,3
]}

Elias, Rony ^{[1
]}

Mihailovic, Aleksandra ^{[2
,3
]}

Brown, Miguel ^{[2
,3
]}

Rosenwaks, Zev ^{[1
]}

Tuschl, Thomas ^{[2
,3
]}

机构：

[1] Weill Cornell Med Ctr, Ctr Reprod Med & Infertil, New York, NY 10021 USA

[2] Rockefeller Univ, Howard Hughes Med Inst, New York, NY 10065 USA

[3] Rockefeller Univ, Lab RNA Mol Biol, New York, NY 10065 USA

[4] Albert Einstein Coll Med, Dept Obstet & Gynecol & Womens Hlth, Bronx, NY 10461 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2013年 / 110卷 / 11期

基金：

美国国家卫生研究院;

关键词：

pregnancy; cancer; EXPRESSION; CANCER; SERUM; IDENTIFICATION; BIOGENESIS; DIAGNOSIS; MIRNAS; TISSUE;

D O I：

10.1073/pnas.1214046110

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We profiled microRNAs (miRNAs) in cell-free serum and plasma samples from human volunteers using deep sequencing of barcoded small RNA cDNA libraries. By introducing calibrator synthetic oligonucleotides during library preparation, we were able to calculate the total as well as specific concentrations of circulating miRNA. Studying trios of samples from newborn babies and their parents we detected placental-specific miRNA in both maternal and newborn circulations and quantitated the relative contribution of placental miRNAs to the circulating pool of miRNAs. Furthermore, sequence variation in the placental miRNA profiles could be traced to the specific placenta of origin. These deep sequencing profiles, which may serve as a model for tumor or disease detection, allow us to define the repertoire of miRNA abundance in the circulation and potential uses as biomarkers.

引用

页码：4255 / 4260

页数：6

共 50 条

[1]

ACOG Committee on Practice Bulletins--Obstetrics, 2002, Obstet Gynecol, V99, P159

[2] MORPHOMETRY OF HUMAN PLACENTA [J].