Covalent immobilization of cholesterol esterase and cholesterol oxidase on polyaniline films for application to cholesterol biosensor

被引：105

作者：

Singh, Surnan ^{[1
]}

Solanki, Pratima R. ^{[1
]}

Pandey, M. K. ^{[1
]}

Malhotra, B. D. ^{[1
]}

机构：

[1] Natl Phys Lab, Biomol Elect & Conducting Polymer Res Grp, New Delhi 110012, India

来源：

ANALYTICA CHIMICA ACTA | 2006年 / 568卷 / 1-2期

关键词：

polyaniline; cholesterol esterase; cholesterol oxidase; covalent immobilization;

D O I：

10.1016/j.aca.2005.10.008

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Cholesterol esterase (ChEt) and cholesterol oxidase (ChOx) have been covalently immobilized on electrochemically prepared polyaniline (PANI) films. These PANI/ChEt/ChOx enzyme films have been characterized using UV-visible, Fourier transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM). Electrochemical behavior of these films has been studied using cyclic voltammetry (CV) and amperometric techniques, respectively. The PANI/ChEt/ChOx enzyme films show broad oxidation peak from 0.2 to 0.5 V. These PANI/ChEt/ChOx biosensing electrodes have a response time of about 40 s, linearity from 50 to 500 mg/dl of cholesterol oleate concentration. These PANI/ChEt/ChOx films are thermally stable up to 46 degrees C. This polyaniline based cholesterol biosensor has optimum pH in the range of 6.5-7.5, sensitivity as 7.5 x 10(-4) nA/mg dl and a lifetime of about 6 weeks. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：126 / 132

页数：7

共 29 条

[1] Improvement of the analytical characteristics of an enzyme electrode for free and total cholesterol via laponite clay additives [J].

Besombes, JL ;

Cosnier, S ;

Labbe, P ;

Reverdy, G .

ANALYTICA CHIMICA ACTA, 1995, 317 (1-3) :275-280

[2] An electrochemical multienzymatic biosensor for determination of cholesterol [J].

Bongiovanni, C ;

Ferri, T ;

Poscia, A ;

Varalli, M ;

Santucci, R ;

Desideri, A .

BIOELECTROCHEMISTRY, 2001, 54 (01) :17-22

[3] The National Cholesterol Education Program - Progress and prospects [J].

Cleeman, JI ;

Lenfant, C .

JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 1998, 280 (24) :2099-2104

[4] SPECTROELECTROCHEMICAL EVIDENCE FOR AN INTERMEDIATE IN THE ELECTROPOLYMERIZATION OF ANILINE [J].

GENIES, EM ;

LAPKOWSKI, M .

JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 1987, 236 (1-2) :189-197

[5] Layer-by-layer construction of an active multilayer enzyme electrode applicable for direct amperometric determination of cholesterol [J].

Gobi, KV ;

Mizutani, F .

SENSORS AND ACTUATORS B-CHEMICAL, 2001, 80 (03) :272-277

[6] CONTACT RESISTANCE MEASUREMENTS RECORDED AT CONDUCTIVE POLYMER HIGH-TEMPERATURE SUPERCONDUCTOR INTERFACES [J].

HAUPT, SG ;

RILEY, DR ;

ZHAO, JN ;

MCDEVITT, JT .

JOURNAL OF PHYSICAL CHEMISTRY, 1993, 97 (30) :7796-7799

[7]

IWUOHA EI, 1996, ELECTROACTIVE POLY 2, P297

[8] CONFERMENT OF CHOLESTEROL SENSITIVITY ON POLYPYRROLE FILMS BY IMMOBILIZATION OF CHOLESTEROL OXIDASE AND FERROCENECARBOXYLATE IONS [J].

KAJIYA, Y ;

TSUDA, R ;

YONEYAMA, H .

JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 1991, 301 (1-2) :155-164

[9]

KILLARD A, INABIS 1998 5 INT WO

[10] FLOW-INJECTION ANALYSIS FOR TOTAL CHOLESTEROL WITH PHOTOMETRIC DETECTION [J].

KRUG, A ;

GOBEL, R ;

KELLNER, R .

ANALYTICA CHIMICA ACTA, 1994, 287 (1-2) :59-64

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