An electrochemical multienzymatic biosensor for determination of cholesterol

被引:69
作者
Bongiovanni, C
Ferri, T
Poscia, A
Varalli, M
Santucci, R
Desideri, A
机构
[1] Univ Roma Tor Vergata, Dipartimento Biol, I-00133 Rome, Italy
[2] Univ Roma Tor Vergata, INFM, I-00133 Rome, Italy
[3] Univ Roma La Sapienza, Dipartimento Chim, I-00185 Rome, Italy
[4] Menarini Ind Farmaceut Riunite SRL, Florence, Italy
[5] Univ Roma Tor Vergata, Dipartimento Med Sperimentale & Sci Biochim, I-00133 Rome, Italy
关键词
cholesterol; biosensor; cyclic voltammetry; flow injection analysis; horseradish peroxidase;
D O I
10.1016/S0302-4598(01)00105-2
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
This paper describes an electrochemical biosensor for free cholesterol monitoring. The sensor is a multienzymatic electrodic system in which horseradish peroxidase and cholesterol oxidase are simultaneously immobilized within a polymeric film. on the surface of a pyrolitic graphite electrode. From voltammetric and amperometric (flow-injection) data obtained, the efficiency, reproducibility and stability of the system are discussed. Results obtained, of interest for basic and applied biochemistry, represent a first step for construction of a mediator-free biosensor with potentialities for a successful application in the biosensor area. (C) 2001 Elsevier Science BN. All rights reserved.
引用
收藏
页码:17 / 22
页数:6
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