Stabilization and Induction of Oligonucleotide i-Motif Structure via Graphene Quantum Dots

DNA i-motif structures have been found in telomeric, centromeric DNA and many in the promoter region of oncogenes; thus they might be attractive targets for gene-regulation processes and anticancer therapeutics. We demonstrate in this work that i-motif structures can be stabilized by graphene quantum dots (GQDs) under acidic conditions, and more importantly GODs can promote the formation of the i-motif structure under alkaline or physiological conditions. We Illustrate that the GQDs stabilize the i-motif structure through end-stacking of the bases at its loop regions, thus reducing its solvent-accessible area. Under physiological or alkaline conditions, the end-stacking of GQDs on the unfolded structure shifts the equilibrium between the I-motif and unfolded structure toward the i-motif structure, thus promoting its formation. The possibility of fine-tuning the stability of the I-motif and Inducing its formation would make GQDs useful in gene regulation and oligonucleotide-based therapeutics.