Chromatographic and spectroscopic assignment of thiol induced cycloaromatizations of enediyne in neocarzinostatin

被引：11

作者：

Chin, DH

Tseng, MC

Chuang, TC

Hong, MC

机构：

[1] Department of Chemistry, Natl. Changhua Univ. of Education, Changhua 50058, Paisa Village

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS | 1997年 / 1336卷 / 01期

关键词：

antitumor drug; neocarzinostatin; cycloaromatization;

D O I：

10.1016/S0304-4165(97)00008-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Neocarzinostatin is an enediyne antitumor antibiotic. Upon attack by a thiol, the enediyne nucleus is cycloaromatized into two stable 1:1 thiol adducts. After analyzing products from various thiols, the chromatographic and spectroscopic characters that associate only with the cyclized aromatic moiety from enediyne nucleus were assigned. Based on HPLC analysis we have derived, products from picomole ranges of the drug sample can be detected. Confirming the type of cycloaromatization at nanomole ranges can be achieved by photodiode array UV spectroscopy. Three-dimensional fluorogram presents ten times more sensitive identification. The method provides a sensitive tool for massive screening study in microscale.

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页码：43 / 50

页数：8

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