Polydopamine-mediated surface modification of scaffold materials for human neural stem cell engineering

被引：409

作者：

Yang, Kisuk ^{[1
]}

Lee, Jung Seung ^{[1
]}

Kim, Jin ^{[1
]}

Lee, Yu Bin ^{[2
]}

Shin, Heungsoo ^{[2
]}

Um, Soong Ho ^{[3
]}

Kim, Jeong Beom ^{[4
]}

Park, Kook In ^{[5
,6
]}

Lee, Haeshin ^{[7
]}

Cho, Seung-Woo ^{[1
]}

机构：

[1] Yonsei Univ, Dept Biotechnol, Seoul 120749, South Korea

[2] Hanyang Univ, Dept Bioengn, Seoul 133791, South Korea

[3] Sungkyunkwan Univ, Sch Chem Engn, Suwon 440746, South Korea

[4] Ulsan Natl Inst Sci & Technol, Sch Nanobiotechnol & Chem Engn, Ulsan 689798, South Korea

[5] Yonsei Univ, Coll Med, Dept Pediat, Seoul 120752, South Korea

[6] Yonsei Univ, Coll Med, Project Med Sci BK21, Seoul 120752, South Korea

[7] Korea Adv Inst Sci & Technol, Dept Chem, Taejon 305701, South Korea

来源：

BIOMATERIALS | 2012年 / 33卷 / 29期

基金：

新加坡国家研究基金会;

关键词：

Polydopamine; Surface immobilization; Neural stem cell; Neurotrophic growth factor; Adhesion peptide; NEURONAL DIFFERENTIATION; IN-VITRO; ENDOTHELIAL-CELLS; PRECURSOR CELLS; GROWTH-FACTOR; TRANSPLANTATION; SURVIVAL; STROKE; PROLIFERATION; VIABILITY;

D O I：

10.1016/j.biomaterials.2012.06.067

中图分类号：

R318 [生物医学工程];

学科分类号：

100103 [病原生物学];

摘要：

Surface modification of tissue engineering scaffolds and substrates is required for improving the efficacy of stem cell therapy by generating physicochemical stimulation promoting proliferation and differentiation of stem cells. However, typical surface modification methods including chemical conjugation or physical absorption have several limitations such as multistep, complicated procedures, surface denaturation, batch-to-batch inconsistencies, and low surface conjugation efficiency. In this study, we report a mussel-inspired, biomimetic approach to surface modification for efficient and reliable manipulation of human neural stem cell (NSC) differentiation and proliferation. Our study demonstrates that polydopamine coating facilitates highly efficient, simple immobilization of neurotrophic growth factors and adhesion peptides onto polymer substrates. The growth factor or peptide-immobilized substrates greatly enhance differentiation and proliferation of human NSCs (human fetal brain-derived NSCs and human induced pluripotent stem cell-derived NSCs) at a level comparable or greater than currently available animal-derived coating materials (Matrigel) with safety issues. Therefore, polydopamine-mediated surface modification can provide a versatile platform technology for developing chemically defined, safe, functional substrates and scaffolds for therapeutic applications of human NSCs. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：6952 / 6964

页数：13

共 40 条

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Neural stem cell adhesion and proliferation on phospholipid bilayers functionalized with RGD peptides [J].