Multifunctional in vivo vascular imaging using near-infrared II fluorescence

被引：934

作者：

Hong, Guosong ^{[1
]}

Lee, Jerry C. ^{[2
]}

Robinson, Joshua T. ^{[1
]}

Raaz, Uwe ^{[2
]}

Xie, Liming ^{[1
]}

Huang, Ngan F. ^{[2
]}

Cooke, John P. ^{[2
]}

Dai, Hongjie ^{[1
]}

机构：

[1] Stanford Univ, Dept Chem, Stanford, CA 94305 USA

[2] Stanford Univ, Sch Med, Div Cardiovasc Med, Stanford, CA 94305 USA

来源：

NATURE MEDICINE | 2012年 / 18卷 / 12期

基金：

美国国家卫生研究院;

关键词：

WALLED CARBON NANOTUBES; QUANTUM DOTS; CELLS; MICE; THICKNESS; TUMORS; MEDIA; CT;

D O I：

10.1038/nm.2995

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

In vivo real-time epifluorescence imaging of mouse hind limb vasculatures in the second near-infrared region (NIR-II) is performed using single-walled carbon nanotubes as fluorophores. Both high spatial (similar to 30 mu m) and temporal (<200 ms per frame) resolution for small-vessel imaging are achieved at 1-3 mm deep in the hind limb owing to the beneficial NIR-II optical window that affords deep anatomical penetration and low scattering. This spatial resolution is unattainable by traditional NIR imaging (NIR-I) or microscopic computed tomography, and the temporal resolution far exceeds scanning microscopic imaging techniques. Arterial and venous vessels are unambiguously differentiated using a dynamic contrast-enhanced NIR-II imaging technique on the basis of their distinct hemodynamics. Further, the deep tissue penetration and high spatial and temporal resolution of NIR-II imaging allow for precise quantifications of blood velocity in both normal and ischemic femoral arteries, which are beyond the capabilities of ultrasonography at lower blood velocities.

引用

页码：1841 / +

页数：8

共 40 条

[1]

Visualization of advanced human prostate cancer lesions in living mice by a targeted gene transfer vector and optical imaging [J].