Recombinant Canine Coronaviruses Related to Transmissible Gastroenteritis Virus of Swine Are Circulating in Dogs

被引:126
作者
Decaro, Nicola [1 ]
Mari, Viviana [1 ]
Campolo, Marco [1 ]
Lorusso, Alessio [1 ]
Camero, Michele [1 ]
Elia, Gabriella [1 ]
Martella, Vito [1 ]
Cordioli, Paolo [2 ]
Enjuanes, Luis [3 ]
Buonavoglia, Canio [1 ]
机构
[1] Fac Vet Med Bari, Dept Publ Hlth & Anim Sci, I-70010 Bari, Italy
[2] Ist Zooprofilatt Sperimentale Lombardia Emilia Ro, I-25124 Brescia, Italy
[3] Campus Univ Autonoma, Dept Mol & Cell Biol, Ctr Nacl Biotecnol, Madrid, Spain
关键词
SUBGENOMIC RNA-SYNTHESIS; BINDER PROBE TECHNOLOGY; TIME PCR ASSAY; MOLECULAR CHARACTERIZATION; PARVOVIRUS TYPE-2; FIELD STRAINS; GLYCOPROTEIN; QUANTITATION; INFECTION;
D O I
10.1128/JVI.01937-08
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Four canine coronavirus type II (CCoV-II) strains were identified in the guts and internal organs of pups which had died of acute gastroenteritis. The CCoV-II strains were strictly related to porcine transmissible gastroenteritis virus (TGEV) in the N-terminal domain of the spike protein, whereas in the other parts of the genome, a higher genetic relatedness to recent CCoV-II isolates was observed. Experimental infection of dogs with a TGEV-like isolate induced mild gastroenteritis without any systemic involvement. By virus neutralization tests, antigenic differences between reference and TGEV-like CCoVs were found. Our data support the potential recombinant origin of the TGEV-like CCoVs.
引用
收藏
页码:15332 / 1537
页数:6
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