Comparison of preparative HPLC/MS and preparative SFC techniques for the high-throughput purification of compound libraries

被引：41

作者：

Searle, PA ^{[1
]}

Glass, KA ^{[1
]}

Hochlowski, JE ^{[1
]}

机构：

[1] Abbott Labs, Dept R4CP, Abbott Pk, IL 60064 USA

来源：

JOURNAL OF COMBINATORIAL CHEMISTRY | 2004年 / 6卷 / 02期

关键词：

D O I：

10.1021/cc0340372

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A diverse set of 16 high-throughput organic synthesis libraries, consisting of 48 samples per library, has been purified by both preparative supercritical fluid chromatography (SFC) and preparative high-performance liquid chromatography (HPLC). This paper details the relative effectiveness of these two purification techniques in terms of success, yield, and purity of final product.

引用

收藏

页码：175 / 180

页数：6

相关论文

共 28 条

[1] The development of a semi-preparatory scale supercritical-fluid chromatograph for high-throughput purification of 'combi-chem' libraries [J].

Berger, TA ;

Fogleman, K ;

Staats, T ;

Bente, P ;

Crocket, I ;

Farrell, W ;

Osonubi, M .

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS, 2000, 43 (1-3) :87-111

[2] High-speed screening of combinatorial libraries by gradient packed-column supercritical fluid chromatography [J].

Berger, TA ;

Wilson, WH .

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS, 2000, 43 (1-3) :77-85

[3]

Berger TA., 1995, Packed Column SFC

[4] Scope and limitations of solid-supported liquid-liquid extraction for the high-throughput purification of compound libraries [J].

Breitenbucher, JG ;

Arienti, KL ;

McClure, KJ .

JOURNAL OF COMBINATORIAL CHEMISTRY, 2001, 3 (06) :528-533

[5] A straightforward means of coupling preparative high-performance liquid chromatography and mass spectrometry [J].

Cai, H ;

Kiplinger, JP ;

Goetzinger, WK ;

Cole, RO ;

Laws, KA ;

Foster, M ;

Schrock, A .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2002, 16 (06) :544-554

[6] Work-up strategies for high-throughput solution synthesis [J].

Cork, D ;

Hird, N .

DRUG DISCOVERY TODAY, 2002, 7 (01) :56-63

[7]

Curran DP, 2001, SYNLETT, P1488

[8] High-throughput purification of combinatorial arrays [J].

Edwards, C ;

Hunter, DJ .

JOURNAL OF COMBINATORIAL CHEMISTRY, 2003, 5 (01) :61-66

[9] Studies of the relative stability of TFA adducts vs non-TFA analogues for combinatorial chemistry library members in DMSO in a repository compound collection [J].

Hochlowski, J ;

Cheng, XH ;

Sauer, D ;

Djuric, S .

JOURNAL OF COMBINATORIAL CHEMISTRY, 2003, 5 (04) :345-349

[10] Purification of HTOS libraries by supercritical fluid chromatography [J].

Hochlowski, J ;

Olson, J ;

Pan, J ;

Sauer, D ;

Searle, P ;

Sowin, T .

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, 2003, 26 (03) :333-354