Identification of antioxidants in Fructus aurantii and its quality evaluation using a new on-line combination of analytical techniques

被引：82

作者：

Lin, Zongtao ^{[1
]}

Wang, Hong ^{[1
]}

Xu, Ying ^{[1
]}

Dong, Jing ^{[2
]}

Hashi, Yuki ^{[2
]}

Chen, Shizhong ^{[1
]}

机构：

[1] Peking Univ, Sch Pharmaceut Sci, Beijing 100191, Peoples R China

[2] Shimadzu Int Trading Shanghai Co Ltd, Beijing 100020, Peoples R China

来源：

FOOD CHEMISTRY | 2012年 / 134卷 / 02期

关键词：

Fructus aurantii; Flavonoids; Antioxidant identification; Quality evaluation; HPLC-DAD-ESI-MS-PCD-LPFCL; IT-TOF; TRAP MASS-SPECTROMETRY; ELECTROSPRAY-IONIZATION; LIQUID-CHROMATOGRAPHY; CITRUS; FLAVONOIDS; HESPERIDIN; EXTRACTS; PEEL;

D O I：

10.1016/j.foodchem.2012.02.161

中图分类号：

O69 [应用化学];

学科分类号：

070301 [无机化学];

摘要：

A new on-line method for simultaneous identification and monitoring of antioxidants in Fructus aurantii was established by coupling high performance liquid chromatography-diode array detector-electrospray ionisation-ion trap-time of flight-mass spectrometry with post-column derivatisation and luminol-potassium ferricyanide chemiluminescence (HPLC-DAD-ESI-IT-TOF-MS-PCD-LPFCL). While the HPLC fingerprint, structural identification and radical scavenging profile were rapidly obtained by an on-line assay using ultraviolet (UV) absorption, MS and LPFCL, details of the precise substitution patterns of various structures were achieved through UV absorption using PCD addition of shift reagents. Twenty-five flavonoids were identified by either their PCD and MS data or comparison with reference substances. Data collected both from chromatograms and activity profiles of 12 samples revealed significant differences among samples from different habitats. The results showed that this method was rapid and precise, and therefore would be an effective and sensitive method for biocompounds analysis and quality evaluation for complex food and medicinal samples. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：1181 / 1191

页数：11

共 27 条

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