Engineered membrane superchannel improves bioremediation potential of dioxin-degrading bacteria

被引：41

作者：

Aso, Y

Miyamoto, Y

Harada, KM

Momma, K

Kawai, S

Hashimoto, W

Mikami, B

Murata, K ^{[1
]}

机构：

[1] Kyoto Univ, Grad Sch Agr, Lab Basic & Appl Mol Biotechnol, Uji, Kyoto 6110011, Japan

[2] Kyoto Univ, Grad Sch Agr, Lab Food Qual Design & Dev, Uji, Kyoto 6110011, Japan

来源：

NATURE BIOTECHNOLOGY | 2006年 / 24卷 / 02期

关键词：

D O I：

10.1038/nbt1181

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Sphingomonas sp. A1 possesses specialized membrane structures termed 'superchannels' that enable the direct incorporation of macromolecules into the cell. We have engineered two related sphingomonads, the dioxin-degrading S. wittichii RW1 and the polypropylene glycol-degrading S. subarctica IFO 16058(T), to incorporate this superchannel into their cell membranes. In both cases the bioremediation capability of the organisms was substantially increased pointing at the potential of this approach as a general strategy to improve bacterial degradation of hazardous compounds in the environment.

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收藏

页码：188 / 189

页数：2

相关论文

共 15 条

[1] Two-liquid-phase bioreactors for enhanced degradation of hydrophobic/toxic compounds [J].

Déziel, E ;

Comeau, Y ;

Villemur, R .

BIODEGRADATION, 1999, 10 (03) :219-233

[2] Molecular identification of oligoalginate lyase of Sphingomonas sp strain A1 as one of the enzymes required for complete depolymerization of alginate [J].

Hashimoto, W ;

Miyake, O ;

Momma, K ;

Kawai, S ;

Murata, K .

JOURNAL OF BACTERIOLOGY, 2000, 182 (16) :4572-4577

[3] Proteomics-based identification of outer-membrane proteins responsible for import of macromolecules in Sphingonionas sp A1:: Alginate-binding flagellin on the cell surface [J].

Hashimoto, W ;

He, JS ;

Wada, Y ;

Nankai, H ;

Mikami, B ;

Murata, K .

BIOCHEMISTRY, 2005, 44 (42) :13783-13794

[4] Structure and function of bacterial super-biosystem responsible for import and depolymerization of macromolecules [J].

Hashimoto, W ;

Momma, K ;

Maruyama, Y ;

Yamasaki, M ;

Mikami, B ;

Murata, K .

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 2005, 69 (04) :673-692

[5] Pit structure on bacterial cell surface [J].

Hisano, T ;

Kimura, N ;

Hashimoto, W ;

Murata, K .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1996, 220 (03) :979-982

[6] Biotransformation of 2,7-dichloro- and 1,2,3,4-tetrachlorodibenzo-p-dioxin by Sphingomonas wittichii RW1 [J].

Hong, HB ;

Chang, YS ;

Nam, IH ;

Fortnagel, P ;

Schmidt, S .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2002, 68 (05) :2584-2588

[7] ISOLATION OF AN UNUSUAL LIPID-A TYPE GLYCOLIPID FROM PSEUDOMONAS-PAUCIMOBILIS [J].

KAWAHARA, K ;

UCHIDA, K ;

AIDA, K .

BIOCHIMICA ET BIOPHYSICA ACTA, 1982, 712 (03) :571-575

[8] CLONING AND SEQUENCING OF 2 TANDEM GENES INVOLVED IN DEGRADATION OF 2,3-DIHYDROXYBIPHENYL TO BENZOIC-ACID IN THE POLYCHLORINATED BIPHENYL-DEGRADING SOIL BACTERIUM PSEUDOMONAS SP STRAIN KKS102 [J].

KIMBARA, K ;

HASHIMOTO, T ;

FUKUDA, M ;

KOANA, T ;

TAKAGI, M ;

OISHI, M ;

YANO, K .

JOURNAL OF BACTERIOLOGY, 1989, 171 (05) :2740-2747

[9] Crystal structure of AlgQ2, a macromolecule (alginate)-binding protein of Sphingomonas sp A1, complexed with an alginate tetrasaccharide at 1.6-A resolution [J].

Mishima, Y ;

Momma, K ;

Hashimoto, W ;

Mikami, B ;

Murata, K .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (08) :6552-6559

[10] A novel bacterial ATP-binding cassette transporter system that allows uptake of macromolecules [J].

Momma, K ;

Okamoto, M ;

Mishima, Y ;

Mori, S ;

Hashimoto, W ;

Murata, K .

JOURNAL OF BACTERIOLOGY, 2000, 182 (14) :3998-4004