Imaging of plant dynamin-related proteins and clathrin around the plasma membrane by variable incidence angle fluorescence microscopy

被引:21
作者
Fujimoto, Masaru [1 ]
Arimura, Shin-ichi [1 ]
Nakazono, Mikio [1 ]
Tsutsumi, Nobuhiro [1 ]
机构
[1] Univ Tokyo, Grad Sch Agr & Life Sci, Plant Mol Genet Lab, Tokyo 1138657, Japan
关键词
Dynamin-related protein; clathrin light chain; variable incidence angle fluorescence microscopy;
D O I
10.5511/plantbiotechnology.24.449
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Endocytosis is an essential phenomenon in eukaryotic cells. In animal cells, dynamin and clathrin play central roles in vesicle formation in the process of endocytosis, but the roles of similar proteins in plants are less well understood. Here, we observed the localization pattern and behavior of GFP-labeled Arabidopsis dynamin-related proteins (DRP1A and DRP2B), and clathrin light chain (AtCLC) around the plasma membrane in tobacco suspension cells by using variable incidence angle fluorescence microscopy (VIAFM). GFP fusions of DRP1A, DRP2B and AtCLC were observed as dot-like puncta 200-500 nm in diameter. The puncta moved to and away from the cell surface or also assembled and disassembled. The localization pattern and behavior of the puncta were similar to those of animal dynamin and clathrin signals reported previously. These results raise the possibility that DRP1A, DRP2B and AtCLC are involved in membrane trafficking around the plasma membrane, including endocytosis.
引用
收藏
页码:449 / 455
页数:7
相关论文
共 38 条
[21]   Nanometer targeting of microtubules to focal adhesions [J].
Krylyshkina, O ;
Anderson, KI ;
Kaverina, I ;
Upmann, I ;
Manstein, DJ ;
Small, JV ;
Toomre, DK .
JOURNAL OF CELL BIOLOGY, 2003, 161 (05) :853-859
[22]   Fate of nascent microtubules organized at the M/G1 interface, as visualized by synchronized tobacco BY-2 cells stably expressing GFP-tubulin:: Time-sequence observations of the reorganization of cortical microtubules in living plant cells [J].
Kumagai, F ;
Yoneda, A ;
Tomida, T ;
Sano, T ;
Nagata, T ;
Hasezawa, S .
PLANT AND CELL PHYSIOLOGY, 2001, 42 (07) :723-732
[23]   Regulation of ADL6 activity by its associated molecular network [J].
Lam, BCH ;
Sage, TL ;
Bianchi, F ;
Blumwald, E .
PLANT JOURNAL, 2002, 31 (05) :565-576
[24]   Endocytosis and molecular sorting [J].
Mellman, I .
ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY, 1996, 12 :575-625
[25]   Imaging actin and dynamin recruitment during invagination of single clathrin-coated pits [J].
Merrifield, CJ ;
Feldman, ME ;
Wan, L ;
Almers, W .
NATURE CELL BIOLOGY, 2002, 4 (09) :691-698
[26]   Endocytotic cycling of PM proteins [J].
Murphy, AS ;
Bandyopadhyay, A ;
Holstein, SE ;
Peer, WA .
ANNUAL REVIEW OF PLANT BIOLOGY, 2005, 56 :221-251
[27]   TOBACCO BY-2 CELL-LINE AS THE HELA-CELL IN THE CELL BIOLOGY OF HIGHER-PLANTS [J].
NAGATA, T ;
NEMOTO, Y ;
HASEZAWA, S .
INTERNATIONAL REVIEW OF CYTOLOGY-A SURVEY OF CELL BIOLOGY, 1992, 132 :1-30
[28]   Three-dimensional analysis of syncytial-type cell plates during endosperm cellularization visualized by high resolution electron tomography [J].
Otegui, MS ;
Mastronarde, DN ;
Kang, BH ;
Bednarek, SY ;
Staehelin, LA .
PLANT CELL, 2001, 13 (09) :2033-2051
[29]   The dynamin superfamily: Universal membrane tubulation and fission molecules? [J].
Praefcke, GJK ;
McMahon, HT .
NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2004, 5 (02) :133-147
[30]   Real-time analysis of clathrin-mediated endocytosis during cell migration [J].
Rappoport, JZ ;
Simon, SM .
JOURNAL OF CELL SCIENCE, 2003, 116 (05) :847-855