Signal amplification by rolling circle amplification on DNA microarrays

被引：156

作者：

Nallur, G ^{[1
]}

Luo, CH ^{[1
]}

Fang, LH ^{[1
]}

Cooley, S ^{[1
]}

Dave, V ^{[1
]}

Lambert, J ^{[1
]}

Kukanskis, K ^{[1
]}

Kingsmore, S ^{[1
]}

Lasken, R ^{[1
]}

Schweitzer, B ^{[1
]}

机构：

[1] Mol Staging Inc, New Haven, CT 06511 USA

来源：

NUCLEIC ACIDS RESEARCH | 2001年 / 29卷 / 23期

关键词：

D O I：

10.1093/nar/29.23.e118

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

While microarrays hold considerable promise in large-scale biology on account of their massively parallel analytical nature, there is a need for compatible signal amplification procedures to increase sensitivity without loss of multiplexing. Rolling circle amplification (RCA) is a molecular amplification method with the unique property of product localization. This report describes the application of RCA signal amplification for multiplexed, direct detection and quantitation of nucleic acid targets on planar glass and gel-coated microarrays. As few as 150 molecules bound to the surface of microarrays can be detected using RCA. Because of the linear kinetics of RCA, nucleic acid target molecules may be measured with a dynamic range of four orders of magnitude. Consequently, RCA is a promising technology for the direct measurement of nucleic acids on microarrays without the need for a potentially biasing preamplification step.

引用

页码：art. no. / e118

页数：9

共 16 条

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