The mammalian central nervous synaptic cleft contains a high density of periodically organized complexes

被引:172
作者
Zuber, B
Nikonenko, I
Klauser, P
Muller, D
Dubochet, J
机构
[1] Univ Lausanne, Lab Anal Ultrastruct, CH-1015 Lausanne, Switzerland
[2] Univ Geneva, Ctr Med Univ, Dept Neuropharmacol, CH-1211 Geneva 4, Switzerland
关键词
cryo-electron microscopy of vitreous section; high-pressure freezing; hippocampus;
D O I
10.1073/pnas.0509527102
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Cryo-electron microscopy of vitreous section makes it possible to observe cells and tissues at high resolution in a close-to-native state. The specimen remains hydrated; chemical fixation and staining are fully avoided. There is minimal molecular aggregation and the density observed in the image corresponds to the density in the object. Accordingly, organotypic hippocampal rat slices were vitrified under high pressure and controlled cryoprotection conditions, cryosectioned at a final thickness of approximate to 70 nm and observed below -170 degrees C in a transmission electron microscope. The general aspect of the tissue compares with previous electron microscopy observations. The detailed analysis of the synapse reveals that the density of material in the synaptic cleft is high, even higher than in the cytoplasm, and that it is organized in 8.2-nm periodic transcleft complexes. Previously undescribed structures of presynaptic and postsynaptic elements are also described.
引用
收藏
页码:19192 / 19197
页数:6
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