Restoration of culturability of starvation-stressed and low-temperature-stressed Escherichia coli O157 cells by using H2O2-degrading compounds

被引:161
作者
Mizunoe, Y [1 ]
Wai, SN [1 ]
Takade, A [1 ]
Yoshida, S [1 ]
机构
[1] Kyushu Univ, Fac Med, Dept Bacteriol, Fukuoka 8128582, Japan
关键词
Escherichia coli O157; nonculturable cells; restoration of culturability; H2O2-degrading compounds;
D O I
10.1007/s002030050741
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Late-exponential-phase cells of Escherichia coli O157:H- strain E32511/HSC became nonculturable in sterilized distilled water microcosms at 4 degrees C. Plate counts declined from 3 x 10(6) to less than 0.1 CFU/ml in about 21 days. However, when samples of microcosms at 21 days were inoculated onto an agar medium amended with catalase or nonenzyme peroxide-degrading compounds such as sodium pyruvate or a-ketoglutaric acid, plate counts increased to 10(4)-10(5) CFU/ml within 48 h. The proposed mode of action of the catalase or pyruvate is via the degradation of the metabolic by-product H2O2, rather than through supplementation of a required nutrient in the recovery of nonculturable cells. Our studies were based on the assumption that E32511/HSC strain responds to starvation and a low temperature by entering a nonculturable state and that the correction of oxidative stress upon the inoculation of bacteria on agar plates promotes recovery of nonculturable cells.
引用
收藏
页码:63 / 67
页数:5
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