The Nrd1-Nab3-Sen1 termination complex interacts with the Ser5-phosphorylated RNA polymerase II C-terminal domain

被引：226

作者：

Vasiljeva, Lidia ^{[1
]}

Kim, Minkyu ^{[1
]}

Mutschler, Hannes ^{[2
]}

Buratowski, Stephen ^{[1
]}

Meinhart, Anton ^{[2
]}

机构：

[1] Harvard Univ, Sch Med, Dept Biol Chem & Mol Pharmacol, Boston, MA 02115 USA

[2] Max Planck Inst Med Res, Dept Biomol Mech, D-69120 Heidelberg, Germany

来源：

NATURE STRUCTURAL & MOLECULAR BIOLOGY | 2008年 / 15卷 / 08期

关键词：

D O I：

10.1038/nsmb.1468

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

RNA polymerase II (Pol II) in Saccharomyces cerevisiae can terminate transcription via several pathways. To study how a mechanism is chosen, we analyzed recruitment of Nrd1, which cooperates with Nab3 and Sen1 to terminate small nucleolar RNAs and other short RNAs. Budding yeast contains three C-terminal domain (CTD) interaction domain (CID) proteins, which bind the CTD of the Pol II largest subunit. Rtt103 and Pcf11 act in mRNA termination, and both preferentially interact with CTD phosphorylated at Ser2. The crystal structure of the Nrd1 CID shows a fold similar to that of Pcf11, but Nrd1 preferentially binds to CTD phosphorylated at Ser5, the form found proximal to promoters. This indicates why Nrd1 cross-links near 5' ends of genes and why the Nrd1-Nab3-Sen1 termination pathway acts specifically at short Pol II-transcribed genes. Nrd1 recruitment to genes involves a combination of interactions with CTD and Nab3.

引用

页码：795 / 804

页数：10

共 61 条

[1] Phosphorylation of serine 2 within the RNA polymerase IIC-terminal domain couples transcription and 3′ end processing [J].

Ahn, SH ;

Kim, M ;

Buratowski, S .

MOLECULAR CELL, 2004, 13 (01) :67-76

[2] Termination of cryptic unstable transcripts is directed by yeast RNA-Binding proteins Nrd1 and Nab3 [J].

Arigo, John T. ;

Eyler, Daniel E. ;

Carroll, Kristina L. ;

Corden, Jeffry L. .

MOLECULAR CELL, 2006, 23 (06) :841-851

[3] Regulation of yeast NRD1 expression by premature transcription termination [J].

Arigo, JT ;

Carroll, KL ;

Ames, JM ;

Corden, JL .

MOLECULAR CELL, 2006, 21 (05) :641-651

[4] Cleavage/polyadenylation factor IA associates with the carboxyl-terminal domain of RNA polymerase II in Saccharomyces cerevisiae [J].

Barillà, D ;

Lee, BA ;

Proudfoot, NJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (02) :445-450

[5] Snapshots of the RNA processing factor SCAF8 bound to different phosphorylated forms of the carboxyl- terminal domain of RNA polymerase II [J].

Becker, Roland ;

Loll, Bernhard ;

Meinhart, Anton .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2008, 283 (33) :22659-22669

[6] RNA polymerase II carboxy-terminal domain phosphorylation is required for cotranscriptional pre-mRNA splicing and 3′-end formation [J].

Bird, G ;

Zorio, DAR ;

Bentley, DL .

MOLECULAR AND CELLULAR BIOLOGY, 2004, 24 (20) :8963-8969

[7] Crystallography & NMR system:: A new software suite for macromolecular structure determination [J].

Brunger, AT ;

Adams, PD ;

Clore, GM ;

DeLano, WL ;

Gros, P ;

Grosse-Kunstleve, RW ;

Jiang, JS ;

Kuszewski, J ;

Nilges, M ;

Pannu, NS ;

Read, RJ ;

Rice, LM ;

Simonson, T ;

Warren, GL .

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 1998, 54 :905-921

[8] Connections between mRNA 3′ end processing and transcription termination [J].

Buratowski, S .

CURRENT OPINION IN CELL BIOLOGY, 2005, 17 (03) :257-261

[9] The CTD code [J].

Buratowski, S .

NATURE STRUCTURAL BIOLOGY, 2003, 10 (09) :679-680

[10] Identification of cis elements directing termination of yeast nonpolyadenylated snoRNA transcripts [J].

Carroll, KL ;

Pradhan, DA ;

Granek, JA ;

Clarke, ND ;

Corden, JL .

MOLECULAR AND CELLULAR BIOLOGY, 2004, 24 (14) :6241-6252

← 1 2 3 4 5 6 7 →