User Configurable Microfluidic Device for Multiplexed Immunoassays Based on DNA-Directed Assembly

被引：30

作者：

Schroeder, Hendrik ^{[1
]}

Adler, Michael ^{[2
]}

Gerigk, Katrin ^{[4
]}

Mueller-Chorus, Birgit ^{[3
]}

Goetz, Friedrich ^{[4
]}

Niemeyer, Christof M. ^{[1
]}

机构：

[1] Tech Univ Dortmund, Fak Chem, D-44227 Dortmund, Germany

[2] Chimera Biotec GmbH, D-44227 Dortmund, Germany

[3] Boehringer Ingelheim microParts GmbH, D-44227 Dortmund, Germany

[4] Fachhsch Gelsenkirchen, D-45877 Gelsenkirchen, Germany

来源：

ANALYTICAL CHEMISTRY | 2009年 / 81卷 / 03期

关键词：

ANTIBODY MICROARRAYS; IMMOBILIZATION; STRATEGIES; ATTACHMENT; LIBRARIES;

D O I：

10.1021/ac802228k

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We present a microfluidic device for multiplexed immunoassays based on DNA-directed immobilization. Because of the versatile building blocks used for this technique, it is possible to build up user configurable protein microarrays within the microfluidic system by means of DNA-directed self-assembly, which can be used for immunoassay applications. We demonstrate the performance of our system by parallel detection of cytokines in a multiplex immunoassay, employing silver deposition labeling and optical read-out.

引用

页码：1275 / 1279

页数：5

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