High-throughput antibody microarrays for quantitative proteomic analysis

被引：27

作者：

Lv, Lin-Li ^{[1
]}

Liu, Bi-Cheng ^{[1
]}

机构：

[1] SE Univ, Zhong Hosp, Inst Nephrol, Nanjing 210009, Peoples R China

来源：

EXPERT REVIEW OF PROTEOMICS | 2007年 / 4卷 / 04期

基金：

中国国家自然科学基金;

关键词：

antibody microarray; disease proteomic; protein microarray; proteomic; quantitation;

D O I：

10.1586/14789450.4.4.505

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The antibody microarray is an intrinsically robust and quantitative system that delivers high-throughput and parallel measurements on particular sets of known proteins. It has become an important proteomics research tool, complementary to the conventional unbiased separation-based and mass spectrometry-based approaches. This review summarizes the technical aspects of production and the application for quantitative proteomic analysis with an emphasis on disease proteomics, especially the identification of biomarkers. Quality control, data analysis methods and the challenges for quantitative assays are also discussed.

引用

页码：505 / 513

页数：9

共 64 条

[1] Profiling of differential protein expression in angiogenin-induced HUVECs using antibody-arrayed ProteoChip [J].

Ahn, EH ;

Kang, DK ;

Chang, SI ;

Kang, CS ;

Han, MH ;

Kang, IC .

PROTEOMICS, 2006, 6 (04) :1104-1109

[2] The roles of multiple proteomic platforms in a pipeline for new diagnostics [J].

Anderson, NL .

MOLECULAR & CELLULAR PROTEOMICS, 2005, 4 (10) :1441-1444

[3] Progress in protein and antibody microarray technology [J].

Angenendt, P .

DRUG DISCOVERY TODAY, 2005, 10 (07) :503-511

[4] Proteomics in clinical trials and practice - Present uses and future promise [J].

Azad, Nilofer S. ;

Rasool, Nabila ;

Annunziata, Christina M. ;

Minasian, Lori ;

Whiteley, Gordon ;

Kohn, Elise C. .

MOLECULAR & CELLULAR PROTEOMICS, 2006, 5 (10) :1819-1829

[5] Quantitative protein profiling using antibody arrays [J].

Barry, R ;

Soloviev, M .

PROTEOMICS, 2004, 4 (12) :3717-3726

[6] Proteomics of breast cancer - Principles and potential clinical applications [J].

Bertucci, Francois ;

Birnbaum, Daniel ;

Goncalves, Anthony .

MOLECULAR & CELLULAR PROTEOMICS, 2006, 5 (10) :1772-1786

[7] Application of immunoproteomics to rapid cytokine detection [J].

Boyle, MDP ;

Hess, JL ;

Nuara, AA ;

Buller, RM .

METHODS, 2006, 38 (04) :342-350

[8] High-throughput antibody production [J].

Chambers, RS .

CURRENT OPINION IN CHEMICAL BIOLOGY, 2005, 9 (01) :46-50

[9] Quantum dot bioconjugates for ultrasensitive nonisotopic detection [J].

Chan, WCW ;

Nie, SM .

SCIENCE, 1998, 281 (5385) :2016-2018

[10] Phosphoprotein analysis: from proteins to proteomes [J].

Delom, Frederic ;

Chevet, Eric .

PROTEOME SCIENCE, 2006, 4 (1)

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