Reversible binding of DNA to mica for AFM imaging

被引：111

作者：

Thomson, NH ^{[1
]}

Kasas, S ^{[1
]}

Smith, B ^{[1
]}

Hansma, HG ^{[1
]}

Hansma, PK ^{[1
]}

机构：

[1] UNIV CALIF SANTA BARBARA,DEPT PHYS,SANTA BARBARA,CA 93106

来源：

LANGMUIR | 1996年 / 12卷 / 24期

关键词：

D O I：

10.1021/la960497j

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Controlling DNA adsorption to atomically fiat surfaces is crucial for studying protein-DNA interactions with the atomic force microscope (AFM). We present a method that enables reversible binding of this molecule to mica during AFM imaging in liquids. This can be acheived using a flow-through system which allows the exchange of different buffers that promote and inhibit the DNA adhesion to the surface. The DNA adsorption is controlled primarily through the divalent cation concentration. The DNA visibility in the AFM was investigated as a function of pH and cation concentration.

引用

页码：5905 / 5908

页数：4

共 23 条

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