Two-dimensional DOSY experiment with Excitation Sculpting water suppression for the analysis of natural and biological media

被引：42

作者：

Balayssac, Stephane ^{[1
]}

Delsuc, Marc-Andre ^{[2
,3
]}

Gilard, Veronique ^{[1
]}

Prigent, Yann

Malet-Martino, Myriam ^{[1
]}

机构：

[1] Univ Toulouse 3, UMR CNRS 5068, Lab SPCMIB, Grp RMN Biomed, F-31062 Toulouse, France

[2] Univ Montpellier I, INSERM U554, UMR CNRS 5048, Ctr Biochim Struct, F-34090 Montpellier, France

[3] Univ Montpellier 2, INSERM U554, UMR CNRS 5048, Ctr Biochim Struct, F-34090 Montpellier, France

来源：

JOURNAL OF MAGNETIC RESONANCE | 2009年 / 196卷 / 01期

关键词：

BPPSTE; Excitation sculpting; Water suppression signal; 2D DOSY; Complex mixtures; NMR-SPECTROSCOPY; FIELD-GRADIENT; DIFFUSION MEASUREMENTS; SOLVENT SUPPRESSION;

D O I：

10.1016/j.jmr.2008.09.022

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The Bipolar Pulse Pair Stimulated Echo NMR pulse sequence was modified to blend the original Excitation Sculpting water signal suppression. The sequence is a powerful tool to generate rapidly, with a good spectrum quality, bidimensional DOSY experiments without solvent signal, thus allowing the analysis of complex mixtures Such as plant extracts or biofluids. The sequence has also been successfully implemented for a protein at very-low concentration in interaction with a small ligand, namely the salivary IB5 protein binding the polyphenol epigallocatechine gallate. The artifacts created by this sequence can be observed, checked and removed thanks to NPK and NMRnotebook softwares to give a perfect bidimensional DOSY spectrum. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：78 / 83

页数：6

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