A set of simple PCR markers converted from sequence specific RFLP markers on tomato chromosomes 9 to 12

被引：22

作者：

Bai, YL ^{[1
]}

Feng, XH ^{[1
]}

van der Hulst, R ^{[1
]}

Lindhout, P ^{[1
]}

机构：

[1] Univ Wageningen & Res Ctr, Grad Sch Expt Plant Sci, Lab Plant Breeding, NL-6700 AJ Wageningen, Netherlands

来源：

MOLECULAR BREEDING | 2004年 / 13卷 / 03期

关键词：

CAPS; Lycopersicon; molecular marker; polymorphism; SCAR;

D O I：

10.1023/B:MOLB.0000022535.82602.79

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

A set of 24 simple PCR markers was generated for tomato chromosomes 9, 10, 11 and 12. Polymorphism was sought for between Lycopersicon esculentum and one of six other Lycopersicon species (L. parviflorum, L. cheesmanii, L. hirsutum, L. pennellii, L. peruvianum, and L. chilense). PCR primers, which were designed from mapped RFLP sequences, were used to amplify genomic DNA of the different species and the PCR amplification products were screened for polymorphism by testing restriction enzymes. With this approach, 24 (71%) of the 34 selected RFLPs were converted into simple PCR markers. By using a reference population, the map positions of these markers relative to the original RFLP markers were verified. These markers are locus specific and can be efficiently used for alignment of linkage maps, mapping target genes and marker assisted selection.

引用

收藏

页码：281 / 287

页数：7

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