Chromosomal assignment of 79 cDNAs from a range of human tissues

被引：2

作者：

Evans, C ^{[1
]}

Bouzyk, M ^{[1
]}

Cox, S ^{[1
]}

Warne, D ^{[1
]}

Bryant, SP ^{[1
]}

Spurr, NK ^{[1
]}

机构：

[1] IMPERIAL CANC RES FUND,CLARE HALL LABS,HUMAN GENET RESOURCES LAB,S MIMMS EN6 3LD,HERTS,ENGLAND

来源：

GENOMICS | 1996年 / 31卷 / 01期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1006/geno.1996.0021

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Single-pass DNA sequencing of cDNAs selected at random from a human mixed tissue cDNA library have generated a series of more than 2000 expressed sequence tags. One hundred twenty-eight unique cDNA fragments with little or no known protein or nucleic acid homologies have been selected for further analysis. Oligonucleotide primer pairs have been designed from the cDNAs and used in PCR amplification in combination with genomic DNA from a panel of monochromosomal somatic cell hybrids. This has allowed us to assign 70 of these transcribed genes to a single chromosome, and a further 9 have been located on two or three chromosomes. Additionally, 3 cDNAs contain short tandem repeats that may allow them to be further localized by linkage analysis. (C) 1996 Academic Press, Inc.

引用

页码：130 / 134

页数：5

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