Maize cytosolic NADP-malic enzyme (ZmCytNADP-ME): a phylogenetically distant isoform specifically expressed in embryo and emerging roots

被引：29

作者：

Detarsio, Enrique ^{[1
]}

Maurino, Veronica G. ^{[2
]}

Alvarez, Clarisa E. ^{[1
]}

Mueller, Gabriela L. ^{[1
]}

Andreo, Carlos S. ^{[1
]}

Drincovich, Maria F. ^{[1
]}

机构：

[1] Univ Nacl Rosario, Ctr Estudios Fotosintet & Bioquim CEFOBI, RA-2000 Rosario, Santa Fe, Argentina

[2] Univ Cologne, Inst Bot, D-50931 Cologne, Germany

来源：

PLANT MOLECULAR BIOLOGY | 2008年 / 68卷 / 4-5期

关键词：

NADP-malic enzyme; Zea mays; Cytosolic; Embryo; Stress response; Malate;

D O I：

10.1007/s11103-008-9375-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two maize plastidic NADP-malic enzyme isoforms have been characterized: the bundle sheath-located photosynthetic isoform (ZmC(4)-NADP-ME) and a constitutively expressed one (Zm-nonC(4)-NADP-ME). In this work, the characterization of the first maize cytosolic NADP-ME (ZmCytNADP-ME) is presented, which transcript is exclusively found in embryo and emerging roots. ZmCytNADP-ME expression in roots decreases with development, while Zm-nonC(4)-NADP-ME increases concomitantly. On the other hand, ZmCytNADP-ME accumulation is differentially modulated by several stress conditions and shows coordination with that of Zm-nonC(4)-NADP-ME in maize young roots. Recombinant ZmCytNADP-ME displays clearly distinct kinetic parameters and metabolic regulation than the plastidic isoforms. The particular properties and the specific-expression pattern of this novel isoform suggest that it may be involved in the control of cytosolic malate levels in emerging roots, e. g. during hypoxia. ZmCytNADP-ME is phylogenetically related to other cytosolic mono and dicot NADP-MEs, and data indicate that it belongs to an ancestral unique group among plant NADP-MEs.

引用

页码：355 / 367

页数：13

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