The AcrAB-TolC efflux system of Salmonella enterica serovar Typhimurium plays a role in pathogenesis

被引:183
作者
Buckley, AM
Webber, MA
Cooles, S
Randall, LP
La Ragione, RM
Woodward, MJ
Piddock, LJV [1 ]
机构
[1] Univ Birmingham, Div Immun & Infect, Antimicrobial Agents Res Grp, Birmingham B15 2TT, W Midlands, England
[2] Vet Labs Agcy Weybridge, Dept Food & Environm Safety, Addlestone KT15 3NB, Surrey, England
关键词
D O I
10.1111/j.1462-5822.2005.00671.x
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The ability of an isogenic set of mutants of Salmonella enterica serovar Typhimurium L354 (SL1344) with defined deletions in genes encoding components of tripartite efflux pumps, including acrB, acrD, acrF and tolC, to colonize chickens was determined in competition with L354. In addition, the ability of L354 and each mutant to adhere to, and invade, human embryonic intestine cells and mouse monocyte macrophages was determined in vitro. The tolC and acrB knockout mutants were hyper-susceptible to a range of antibiotics, dyes and detergents; the tolC mutant was also more susceptible to acid pH and bile and grew more slowly than L354. Complementation of either gene ablated the phenotype. The tolC mutant poorly adhered to both cell types in vitro and was unable to invade macrophages. The acrB mutant adhered, but did not invade macrophages. In vivo, both the acrB mutant and the tolC mutant colonized poorly and did not persist in the avian gut, whereas the acrD and acrF mutant colonized and persisted as well as L354. These data indicate that the AcrAB-TolC system is important for the colonization of chickens by S. Typhimurium and that this system has a role in mediating adherence and uptake into target host cells.
引用
收藏
页码:847 / 856
页数:10
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