The Rev protein is able to transport to the cytoplasm small nucleolar RNAs containing a Rev binding element

被引:18
作者
Buonomo, SBC [1 ]
Michienzi, A [1 ]
De Angelis, FG [1 ]
Bozzoni, I [1 ]
机构
[1] Univ Rome La Sapienza, Dipt Genet & Biol Mol Edificio Fisiol Generale, Ist Pasteur, Fdn Cenci Bolognetti, I-00185 Rome, Italy
关键词
decoy; nucleolus; oocyte; transport; Xenopus laevis;
D O I
10.1017/S1355838299990064
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Small nucleolar RNAs (snoRNAs) were utilized to express Rev-binding sequences inside the nucleolus and to test whether they are substrates for Rev binding and transport, We show that U16 snoRNA containing the minimal binding site for Rev stably accumulates inside the nucleolus maintaining the interaction with the basic C/D snoRNA-specific factors. Upon Rev expression, the chimeric RNA is exported to the cytoplasm, where it remains bound to Rev in a particle devoid of snoRNP-specific factors, These data indicate that Rev can elicit the functions of RNA binding and transport inside the nucleolus.
引用
收藏
页码:993 / 1002
页数:10
相关论文
共 53 条
  • [1] ANTISENSE SNORNAS - A FAMILY OF NUCLEOLAR RNAS WITH LONG COMPLEMENTARITIES TO RIBOSOMAL-RNA
    BACHELLERIE, JP
    MICHOT, B
    NICOLOSO, M
    BALAKIN, A
    NI, JW
    FOURNIER, MJ
    [J]. TRENDS IN BIOCHEMICAL SCIENCES, 1995, 20 (07) : 261 - 264
  • [2] Transduction of human CD34(+) hematopoietic progenitor cells by a retroviral vector expressing an RRE decoy inhibits human immunodeficiency virus type 1 replication in myelomonocytic cells produced in long-term culture
    Bahner, I
    Kearns, K
    Hao, QL
    Smogorzewska, EM
    Kohn, DB
    [J]. JOURNAL OF VIROLOGY, 1996, 70 (07) : 4352 - 4360
  • [3] The RNA world of the nucleolus: Two major families of small RNAs defined by different box elements with related functions
    Balakin, AG
    Smith, L
    Fournier, MJ
    [J]. CELL, 1996, 86 (05) : 823 - 834
  • [4] HIV-1 REV REGULATION INVOLVES RECOGNITION OF NON-WATSON-CRICK BASE-PAIRS IN VIRAL-RNA
    BARTEL, DP
    ZAPP, ML
    GREEN, MR
    SZOSTAK, JW
    [J]. CELL, 1991, 67 (03) : 529 - 536
  • [5] AN INTACT BOX-C SEQUENCE IN THE U3 SNRNA IS REQUIRED FOR BINDING OF FIBRILLARIN, THE PROTEIN COMMON TO THE MAJOR FAMILY OF NUCLEOLAR SNRNPS
    BASERGA, SJ
    YANG, XDW
    STEITZ, JA
    [J]. EMBO JOURNAL, 1991, 10 (09) : 2645 - 2651
  • [6] In vivo identification of nuclear factors interacting with the conserved elements of box C/D small nucleolar RNAs
    Caffarelli, E
    Losito, M
    Giorgi, C
    Fatica, A
    Bozzoni, I
    [J]. MOLECULAR AND CELLULAR BIOLOGY, 1998, 18 (02) : 1023 - 1028
  • [7] Processing of the intron-encoded U16 and U18 snoRNAs: The conserved C and D boxes control both the processing reaction and the stability of the mature snoRNA
    Caffarelli, E
    Fatica, A
    Prislei, S
    DeGregorio, E
    Fragapane, P
    Bozzoni, I
    [J]. EMBO JOURNAL, 1996, 15 (05) : 1121 - 1131
  • [8] THE HIV-1 REV PROTEIN - PROTOTYPE OF A NOVEL CLASS OF EUKARYOTIC POSTTRANSCRIPTIONAL REGULATORS
    CULLEN, BR
    MALIM, MH
    [J]. TRENDS IN BIOCHEMICAL SCIENCES, 1991, 16 (09) : 346 - 350
  • [9] DUNDR M, 1995, J CELL SCI, V108, P2811
  • [10] FEEDBACK-REGULATION OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 EXPRESSION BY THE REV PROTEIN
    FELBER, BK
    DRYSDALE, CM
    PAVLAKIS, GN
    [J]. JOURNAL OF VIROLOGY, 1990, 64 (08) : 3734 - 3741