Superoxide dismutase biosensors for superoxide radical analysis

被引:43
作者
Campanella, L [1 ]
Favero, G [1 ]
Tomassetti, M [1 ]
机构
[1] Univ Rome La Sapienza, Dept Chem, I-00185 Rome, Italy
关键词
biosensors; superoxide radical; analysis; scavengers;
D O I
10.1080/00032719908542988
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Some basic work has been performed on the development and optimisation of superoxide dismutase (SOD) biosensors for superoxide radical analysis. Initially we studied the possibility of obtaining a SOD biosensor using the Clark electrode as indicating sensor. However, the best results were obtained using as indicator a classical amperometric electrode for H2O2 In both cases the superoxide radical was generated in situ using the xanthine/xanthine oxidase (XOD) enzyme system, while the SOD was immobilised in kappa-carrageenan gel. The first application was realised by studying the effects in vitro on the superoxide radical of some molecules commonly accepted as radical scavengers.
引用
收藏
页码:2559 / 2581
页数:23
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