Expression of toll-like receptors on B lymphocytes

被引:97
作者
Dasari, P
Nicholson, IC
Hodge, G
Dandie, GW
Zola, H
机构
[1] Child Hlth Res Inst, Adelaide, SA 5006, Australia
[2] Womens & Childrens Hosp, Dept Haematol, Adelaide, SA, Australia
[3] Univ Adelaide, Dept Paediat, Adelaide, SA, Australia
关键词
lymphocytes; toll-like receptors;
D O I
10.1016/j.cellimm.2005.08.020
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Toll-like receptors (TLRs) are a family of trans-membrane receptors that play an important role in the innate immune system. Most studies examining the cellular expression of TLRs on immune cells have focussed on neutrophils, monocytes and dendritic cells, but there is little evidence of TLRs being expressed on lymphocytes. Using 3-colour flow cytometry, expression of TLR-1, TLR-2, TLR-3, TLR-4, and TLR-9 on peripheral blood lymphocyte populations was determined. Further examination of TLRs on CD5- and CD5(+) CD19(+) B cell subsets was performed. The binding of TLR1 and TLR9 antibodies was detected on 15-90% of resting B cells, but not on resting T-cells. The higher expression of TLR1 and TLR9 on CD5(+) B cells compared to CD5- B cells may reflect the role of B1 cells in more primitive, less specific antibody responses. (c) 2005 Elsevier Inc. All rights reserved.
引用
收藏
页码:140 / 145
页数:6
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