The RPG gene of Medicago truncatula controls Rhizobium-directed polar growth during infection

被引:90
作者
Arrighi, Jean-Francois
Godfroy, Olivier
de Billy, Francoise
Saurat, Olivier
Jauneau, Alain
Gough, Clare [1 ]
机构
[1] Ctr Natl Rech Sci, Lab Interact Plantes Microorganismes, Unite Mixte Rech 441 2594, Inst Natl Rech Agron, F-31326 Castanet Tolosan, France
[2] Inst Fed Rech 40, Pole Rech Biotechnol Vegetale, F-31326 Castanet Tolosan, France
[3] Univ Aix Marseille, Lab Biol Dev Plantes, Unite Mixte Rech 6191, Ctr Natl Rech Commissariat Energie Atom, F-13108 St Paul Les Durance, France
关键词
genetics; symbiosis; coiled-coil;
D O I
10.1073/pnas.0710273105
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Rhizobia can infect roots of host legume plants and induce new organs called nodules, in which they fix atmospheric nitrogen. Infection generally starts with root hair curling, then proceeds inside newly formed, intracellular tubular structures called infection threads. A successful symbiotic interaction relies on infection threads advancing rapidly at their tips by polar growth through successive cell layers of the root toward developing nodule primordia. To identify a plant component that controls this tip growth process, we characterized a symbiotic mutant of Medicago truncatula, called rpg for rhizobium-directed polar growth. in this mutant, nitrogen-fixing nodules were rarely formed due to abnormally thick and slowly progressing infection threads. Root hair curling was also abnormal, indicating that the RPG gene fulfils an essential function in the process whereby rhizobia manage to dominate the process of induced tip growth for root hair infection. Map-based cloning of RPG revealed a member of a previously unknown plant-specific gene family encoding putative long coiled-coil proteins we have called RRPs (RPG-related proteins) and characterized by an "RRP domain" specific to this family. RPG expression was strongly associated with rhizobial infection, and the RPG protein showed a nuclear localization, indicating that this symbiotic gene constitutes an important component of symbiotic signaling.
引用
收藏
页码:9817 / 9822
页数:6
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