Flow injection analysis with multiple on-line spectroscopic analysis (UV, IR, 1H-NMR and MS)

Studies on the capabilities of flow injection analysis (FIA) combined with on-line characterisation of model compounds via a combination of diode array UV, H-1-NMR, FT-IR spectroscopy and mass spectrometry are described. Using this combination of spectrometers enabled the on-flow collection of UV, H-1-NMR, IR and MS for a range of model compounds. Samples were introduced into the system as solutions in deuterium oxide in concentrations ranging from 1.4 to 8.4 mg ml(-1). A sample volume of 100 mul was used for FIA at a flow rate of 1 ml min(-1). From these studies a practical working quantity of ca. 140 mug/sample of analyte was determined which provided characteristic spectra. (C) 2002 Elsevier Science B.V. All rights reserved.