Near infrared fluorescence-based bacteriophage particles for ratiometric pH imaging

被引:109
作者
Hilderbrand, Scott A. [1 ]
Kelly, Kimberly A. [1 ]
Niedre, Mark [1 ]
Weissleder, Ralph [1 ,2 ]
机构
[1] Harvard Univ, Sch Med, Massachusetts Gen Hosp, Ctr Mol Imaging Res, Cambridge, MA 02138 USA
[2] Harvard Univ, Sch Med, Massachusetts Gen Hosp, Ctr Syst Biol, Cambridge, MA 02138 USA
关键词
D O I
10.1021/bc800188p
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Fluorogenic imaging agents emitting in the near-infrared are becoming important research tools for disease investigation in vivo. Often pathophysiological states such as cancer and cystic fibrosis are associated with disruptions in acid/base homeostasis. The development of optical sensors for pH imaging would facilitate the investigation of these diseased conditions. In this report, the design and synthesis of a ratiometric near-infrared emitting probe for pH quantification is detailed. The pH-responsive probe is prepared by covalent attachment of pH-sensitive and pH-insensitive fluorophores to a bacteriophage particle scaffold. The pH-responsive cyanine dye, HCyC-646, used to construct the probe, has a fluorogenic pK(a) of 6.2, which is optimized for visualization of acidic pH often associated with tumor hypoxia and other diseased states. Incorporation of pH-insensitive reference dyes enables the ratiometric determination of pH independent of the probe concentration. With the pH-responsive construct, measurement of intracellular pH and accurate determination of pH through optically diffuse biological tissue is demonstrated.
引用
收藏
页码:1635 / 1639
页数:5
相关论文
共 34 条
  • [1] Biodegradable pH-sensing dendritic nanoprobes for near-infrared fluorescence lifetime and intensity imaging
    Almutairi, Adah
    Guillaudeu, Steven J.
    Berezin, Mikhail Y.
    Achilefu, Samuel
    Frechet, Jean M. J.
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2008, 130 (02) : 444 - +
  • [2] INTRACELLULAR PH MEASUREMENT USING SINGLE EXCITATION-DUAL EMISSION FLUORESCENCE RATIOS
    BASSNETT, S
    REINISCH, L
    BEEBE, DC
    [J]. AMERICAN JOURNAL OF PHYSIOLOGY, 1990, 258 (01): : C171 - C178
  • [3] Core/shell fluorescent silica nanopartictes for chemical sensing: Towards single-particle laboratories
    Burns, A
    Sengupta, P
    Zedayko, T
    Baird, B
    Wiesner, U
    [J]. SMALL, 2006, 2 (06) : 723 - 726
  • [4] Abnormal surface liquid pH regulation by cultured cystic fibrosis bronchial epithelium
    Coakley, RD
    Grubb, BR
    Paradiso, AM
    Gatzy, JT
    Johnson, LG
    Kreda, SM
    O'Neal, WK
    Boucher, RC
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (26) : 16083 - 16088
  • [5] pH-sensitive cyanine dyes for biological applications
    Cooper, ME
    Gregory, S
    Adie, E
    Kalinka, S
    [J]. JOURNAL OF FLUORESCENCE, 2002, 12 (3-4) : 425 - 429
  • [6] DEMAS JN, 1971, J PHYS CHEM-US, V75, P991, DOI 10.1021/j100678a001
  • [7] PH imaging
    Gillies, RJ
    Raghunand, N
    Garcia-Martin, ML
    Gatenby, RA
    [J]. IEEE ENGINEERING IN MEDICINE AND BIOLOGY MAGAZINE, 2004, 23 (05): : 57 - 64
  • [8] Gillies Robert J., 1999, Neoplasia (New York), V1, P197, DOI 10.1038/sj.neo.7900037
  • [9] INTRACELLULAR PH IN SINGLE MOTILE CELLS
    HEIPLE, JM
    LANSINGTAYLOR, D
    [J]. JOURNAL OF CELL BIOLOGY, 1980, 86 (03) : 885 - 890
  • [10] Monofunctional near-infrared fluorochromes for imaging applications
    Hilderbrand, SA
    Kelly, KA
    Weissleder, R
    Tung, CH
    [J]. BIOCONJUGATE CHEMISTRY, 2005, 16 (05) : 1275 - 1281