Atypical prion protein in sheep brain collected during the British scrapie-surveillance programme

被引:78
作者
Everest, SJ
Thorne, L
Barnicle, DA
Edwards, JC
Elliott, H
Jackman, R
Hope, J
机构
[1] Vet Labs Agcy Lasswade, Penicuik EH26 0PZ, Midlothian, Scotland
[2] Vet Labs Agcy, Dept TSE Mol Biol, Addlestone KT15 3NB, Surrey, England
[3] Inst Anim Hlth, Pirbright Lab, Woking, Surrey, England
关键词
D O I
10.1099/vir.0.81539-0
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Scrapie of sheep and goats is the most common prion disease (or transmissible spongiform encephalopathy, TSE) of mammals and aggregates of abnormal, proteinase-resistant prion protein (PrPSc) are found in all naturally occurring prion diseases. During active surveillance of British sheep for TSEs, 29 201 sheep brain stem samples were collected from abattoirs and analysed for the presence of PrPSc. Of these samples, 54 were found to be positive by using an ELISA screening test, but 28 of these could not be confirmed initially by immunohistochemistry. These unconfirmed or atypical cases were generally found in PrP genotypes normally associated with relative resistance to clinical scrapie and further biochemical analysis revealed that they contained forms of PrPSc with a relatively protease-sensitive amyloid core, some resembling those of Nor98 scrapie. The presence of these atypical forms of protease-resistant PrP raises concerns that some TSE disorders of PrP metabolism previously may have escaped identification in the British sheep population.
引用
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页码:471 / 477
页数:7
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