Genetic Code Supports Targeted Insertion of Two Amino Acids by One Codon

被引：79

作者：

Turanov, Anton A. ^{[1
,2
]}

Lobanov, Alexey V. ^{[1
,2
]}

Fomenko, Dmitri E. ^{[1
,2
]}

Morrison, Hilary G. ^{[3
]}

Sogin, Mitchell L. ^{[3
]}

Klobutcher, Lawrence A. ^{[4
]}

Hatfield, Dolph L. ^{[5
]}

Gladyshev, Vadim N. ^{[1
,2
]}

机构：

[1] Univ Nebraska, Dept Biochem, Lincoln, NE 68588 USA

[2] Univ Nebraska, Redox Biol Ctr, Lincoln, NE 68588 USA

[3] Marine Biol Lab, Josephine Bay Paul Ctr, Woods Hole, MA 02543 USA

[4] Univ Connecticut, Ctr Hlth, Dept Mol Microbial & Struct Biol, Farmington, CT 06032 USA

[5] NCI, Mol Biol Selenium Sect, Lab Canc Prevent, Ctr Canc Res,NIH, Bethesda, MD 20892 USA

来源：

SCIENCE | 2009年 / 323卷 / 5911期

基金：

美国国家科学基金会;

关键词：

SEQUENCE ELEMENT; TRANSFER-RNA; EXPRESSION;

D O I：

10.1126/science.1164748

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Strict one- to- one correspondence between codons and amino acids is thought to be an essential feature of the genetic code. However, we report that one codon can code for two different amino acids with the choice of the inserted amino acid determined by a specific 3 ' untranslated region structure and location of the dual- function codon within the messenger RNA ( mRNA). We found that the codon UGA specifies insertion of selenocysteine and cysteine in the ciliate Euplotes crassus, that the dual use of this codon can occur even within the same gene, and that the structural arrangements of Euplotes mRNA preserve location- dependent dual function of UGA when expressed in mammalian cells. Thus, the genetic code supports the use of one codon to code for multiple amino acids.

引用

页码：259 / 261

页数：3

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