Efficiency of the polymerase chain reaction amplification of the uid gene for detection of Escherichia coli in contaminated water

被引：31

作者：

Iqbal, S

Robinson, J

Deere, D

Saunders, JR

Edwards, C

Porter, J

机构：

[1] NATL INST BIOTECHNOL & GENET ENGN, FAISALABAD, PAKISTAN

[2] UNIV LIVERPOOL, DEPT GENET & MICROBIOL, LIVERPOOL L69 3BX, MERSEYSIDE, ENGLAND

来源：

LETTERS IN APPLIED MICROBIOLOGY | 1997年 / 24卷 / 06期

关键词：

D O I：

10.1046/j.1472-765X.1997.00160.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Direct detection of Escherichia coli from polluted river water was achieved using polymerase chain reaction (PCR) amplification of the uid gene, Amplification using DNA from environmental samples resulted in non-specific DNA fragments, Specific amplification was achieved through use of the touch-down PCR procedure, Targeting the uidA structural region of the gene gave reproducibly better amplification than targeting the uidR regulatory region. The data demonstrate conditions for optimal specific detection.

引用

页码：498 / 502

页数：5

共 15 条

[1] DETECTION OF ESCHERICHIA-COLI AND SHIGELLA SPP IN WATER BY USING THE POLYMERASE CHAIN-REACTION AND GENE PROBES FOR UID [J].